Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress
Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress
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DOI：
10.1007/s10695-016-0227-1
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发表时间：
2016-04
影响因子：
2.9
通讯作者：
Lin Zeng;Yong-Hong Wang;C. Ai;Jia-Lang Zheng;Chang-wen Wu;Rong Cai
中科院分区：
文献类型：
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作者：
Lin Zeng;Yong-Hong Wang;C. Ai;Jia-Lang Zheng;Chang-wen Wu;Rong Cai
本研究旨在通过测定大黄鱼肝脏中ROS的产生、能量代谢酶（PK、F-ATPase、SDH和MDH）的活性和mRNA水平以及HIF-1α的基因表达，探讨β-葡聚糖对急性缺氧诱导的氧化应激及能量代谢的影响。分别于6、4和2天分别注射0或5 mg kg - 1体重的β-葡聚糖，然后分别暴露于1.5和7.0 mg DO L - 1中48 h。结果表明，β-葡聚糖在致死性缺氧应激中提高了鱼的存活率，减少了活性氧，表明β-葡聚糖可以改善缺氧诱导的氧化应激。结果还表明，β-葡聚糖可以上调PK活性和mRNA水平，表明β-葡聚糖增加了厌氧糖酵解能力。此外，还观察到能量代谢酶基因的协同转录调节，表明HIF-1α是调节这些基因所必需的。综上所述，β-葡聚糖可通过增强大黄鱼的厌氧糖酵解能力来缓解缺氧诱导的氧化应激，强调了转录因子HIF-1α在这一过程中的核心作用。
The aim of the present study was to evaluate the effect of β-glucan on acute hypoxia-induced oxidative stress and the changes in energy metabolism by determining ROS production, activities and mRNA levels of energy metabolism enzyme (PK, F-ATPase, SDH and MDH), and in gene expression of HIF-1α in the liver of large yellow croaker. Fish were injected with β-glucan at a dose of 0 or 5 mg kg−1body weight on 6, 4 and 2 days before exposed to 1.5 and 7.0 mg DO L−1for 48 h. The results showed that β-glucan enhanced survival rate and reduced ROS during the lethal hypoxic stress, indicating that β-glucan could ameliorate hypoxia-induced oxidative stress. Obtained results also showed that β-glucan could up-regulate activities and mRNA levels of PK, demonstrating that β-glucan increased anaerobic glycolysis capacity. Furthermore, a coordinated transcriptional regulation of energy metabolism enzyme genes was observed, suggesting that HIF-1α is required for regulating these genes. In conclusion, β-glucan could alleviate cute hypoxia-induced oxidative stress in large yellow croker by enhancing anaerobic glycolysis capacity, emphasizing a central role of transcription factor HIF-1α in the process.