MAINTENANCE AND GROWTH OF HYMENOLEPIS MICROSTOMA (CESTODA - CYCLOPHYLLIDEA) IN VITRO
MAINTENANCE AND GROWTH OF HYMENOLEPIS MICROSTOMA (CESTODA - CYCLOPHYLLIDEA) IN VITRO
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DOI：
10.2307/3276588
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发表时间：
1967-01-01
影响因子：
1.3
通讯作者：
BERNTZEN, AK
中科院分区：
文献类型：
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作者：
DERYCKE, PH;BERNTZEN, AK
小口膜膜绦虫已在体外从囊虫期生长到幼绦虫。从培养基115 （Berntzen和Mueller， 1964）的原液中建立了基础培养基HM 67，由6%组分A， 28%组分B:C:D:E:F (20:20:20: 5; v:v), 50% Earle' s + 0.5%葡萄糖和16%蒸馏水组成。在空气条件下，培养基HM 67 + 30%血清（pH 7.5），蠕虫的平均长度从0.160 mm增长到0.950 mm。添加1%的全仓鼠胆汁可进一步生长至平均长度4.75 mm。培养的蠕虫末端原体包括正常外观的睾丸、精囊、精囊、卷云和卵巢。
Hymenolepis microstoma has been grown in vitro from the cysticercoid stage to the young tapeworm. A basal medium HM 67 has been established from stock solutions of Medium 115 (Berntzen and Mueller, 1964) and is composed of 6% component A, 28% component B:C:D:E:F (20:20:20:20:5; v:v), 50% Earle''s + 0.5% glucose, and 16% distilled water. With Medium HM 67 + 30% serum (pH 7.5) under air, worms grew from 0.160 mm to an average length of 0.950 mm. The addition of 1% whole hamster bile resulted in further growth to an average length of 4.75 mm. Terminal proglottids of cultured worms contained normal appearing testes, seminal vesicle, seminal receptacle, cirrus, and ovary.