The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation
The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation
复制标题
DOI：
10.1016/j.bbrc.2019.03.178
复制
发表时间：
2019-05-21
影响因子：
3.1
通讯作者：
Maier, Jeanette A. M.
中科院分区：
文献类型：
--
作者：
Castiglioni, Sara;Romeo, Valentina;Maier, Jeanette A. M.
镁转运蛋白TRPM 7和MagT 1在成骨细胞生成中过表达。我们已经证明，沉默TRPM 7或MagT 1加速人骨髓间充质干细胞的成骨分化。在这里，我们证明了TRPM 7和MagT 1的同时下调抑制细胞生长并激活自噬，这是成骨细胞生成早期所需的。在TRPM 7/MagT 1下调细胞中，在存在和不存在成骨刺激物的情况下，激活成骨所需的两种转录因子即RUNX 2和OSTERIX的表达均比对照中诱导得更多，而CODA 1在共沉默细胞中与对照中一样上调。这解释了为什么我们没有发现钙沉积的差异。我们的结论是，两个转运蛋白之一应表达，以加速成骨分化。(C)2019爱思唯尔公司All rights reserved.
The magnesium transporters TRPM7 and MagT1 are overexpressed in osteoblastogenesis. We have shown that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 downregulating cells the expression of two transcription factors required for activating osteogenesis, i.e. RUNX2 and OSTERIX, is induced more than in the controls both in the presence and in the absence of osteogenic stimuli, while CODA1 is upregulated in co-silencing cells as much as in the controls. This explains why we found no differences in calcium deposition. We conclude that one of the two transporters should be expressed to accelerate osteogenic differentiation. (C) 2019 Elsevier Inc. All rights reserved.