Effects of Icariin on insulin resistance via the activation of AMPK pathway in C2C12 mouse muscle cells
Effects of Icariin on insulin resistance via the activation of AMPK pathway in C2C12 mouse muscle cells
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DOI：
10.1016/j.ejphar.2015.03.059
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发表时间：
2015-07-05
影响因子：
5
通讯作者：
Park, Yong-Ki
中科院分区：
文献类型：
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作者：
Han, Yunkyung;Jung, Hyo Won;Park, Yong-Ki
骨骼肌中的胰岛素抵抗是2型糖尿病（T2 D）发展的主要危险因素。本研究探讨淫羊藿苷对C2 C12小鼠骨骼肌细胞胰岛素抵抗的影响。将C2 C12成肌细胞分化成肌管5天，然后在100 nM胰岛素存在下用淫羊藿苷（50和100 nM）或二甲双胍（1 nM）处理24小时。ELISA法检测培养液中脂联素的生成，Western blot法检测AMPK/胰岛素信号通路的激活情况。淫羊藿苷显著增加C2 C12肌管中脂联素的产生。此外，淫羊藿苷还能显著促进AMPK和乙酰辅酶A羧化酶（ACC）的磷酸化。淫羊藿苷上调C2 C12肌管磷脂酰肌醇3-激酶（PI 3 K）的表达和胰岛素受体底物-1（IRS-1）的磷酸化。这些结果表明，淫羊藿苷通过调节骨骼肌中的胰岛素抵抗具有治疗T2 D的治疗潜力。(C)2015爱思唯尔B. V.保留所有权利。
Insulin resistance in skeletal muscle is a major risk factor for the development of type 2 diabetes (T2D). In this study, we investigated the effect of icariin on insulin resistance in C2C12 mouse skeletal muscle cells. C2C12 myoblasts were differentiated into myotubes for five days, then treated with icariin (50 and 100 LIM) or metformin (1 rnM) in the presence of 100 nM insulin for 24 h. Adiponectin production was measured in culture media by ELISA, and AMP-activated protein kinase (AMPK)/insulin signaling pathway activation was assessed by the westem blot analysis. Icariin significantly increased adiponectin production in C2C12 myotubes. Moreover, icariin markedly promoted the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC). Icariin up-regulated the expression of phosphatidylinositol 3-kinase (PI3K) and the phosphorylation of insulin receptor substrate-1 (IRS-1) in C2C12 myotubes. These results suggest that icariin has therapeutic potential for the treatment of T2D via the regulation of insulin resistance in skeletal muscle. (C) 2015 Elsevier B.V. All rights reserved.