Silver Nanostructures for Fluorescence Correlation Spectroscopy: Reduced Volumes and Increased Signal Intensities.
Silver Nanostructures for Fluorescence Correlation Spectroscopy: Reduced Volumes and Increased Signal Intensities.
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DOI：
10.1021/jz301229m
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发表时间：
2012-10-04
期刊：
影响因子：
--
通讯作者：
Lakowicz JR
中科院分区：
文献类型：
--
作者：
Choudhury SD;Ray K;Lakowicz JR
荧光相关光谱（FCS）是一种广泛使用的技术，用于研究微摩尔浓度以下分子的相互作用和动力学。银纳米结构（AgNS）基底可以将FCS的适用性扩展到更高的浓度，这对于许多生物相关反应是有用的。此外，这些底物可以通过增加荧光信号强度来提高检测效率。与先前研究的通过自上而下的纳米纤维制备的材料相比，AgNS基底的制备容易，预计将使它们易于获得并适用于各种FCS应用。
Fluorescence correlation spectroscopy (FCS) is a widely used technique to investigate the interactions and dynamics of molecules, below micromolar concentrations. Silver nanostructure (AgNS) substrates can extend the applicability of FCS to higher concentrations, which is useful for many biologically relevant reactions. Additionally, these substrates can improve detection efficiency by increasing fluorescence signal intensities. The ease of preparation of the AgNS substrates in comparison to previously investigated materials prepared by top-down nanofabrication is expected to make them readily available and suitable for various FCS applications.