Impact of gut permeability on the breast microbiome using a non-human primate model.
Impact of gut permeability on the breast microbiome using a non-human primate model.
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DOI：
10.1017/gmb.2022.9
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Cook, Katherine Loree
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文献类型：
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作者：
Bawaneh, Alaa;Shively, Carol A;Cook, Katherine Loree
我们先前在非人类灵长类动物（NHP）中证明，地中海饮食消费改变了乳房和肠道中乳酸杆菌的比例丰度。这些数据强调了肠道-乳房微生物组相互连接的潜在联系。为了解决这个问题，我们比较了NHP研究中匹配的乳腺和粪便样本中鉴定的细菌种群。饮食模式同时改变两个物种在这两个地区，链球菌lutetiensis和瘤胃球菌扭矩。虽然我们观察到乳房和肠道中乳酸杆菌丰度的相似趋势，但每个地区确定的菌种不同;地中海饮食增加了乳房中的乳酸杆菌_未指明菌种，但调节了乳酸杆菌。animalis和L.我们还研究了肠道通透性对乳腺微生物组的影响。无论饮食模式如何，表现出肠道渗透性生理指标增加（血浆脂多糖升高，绒毛长度减少和杯状细胞减少）的受试者表现出显着不同的乳腺微生物组。肠道屏障功能障碍与乳腺组织中α多样性增加和β多样性显著不同相关。总之，我们的数据支持存在受饮食影响的乳房微生物组，其在很大程度上与肠道微生物组群体不同，但对肠道渗透性敏感。
We previously demonstrated in non-human primates (NHP) that Mediterranean diet consumption shifted the proportional abundance of Lactobacillus in the breast and gut. This data highlights a potential link about gut-breast microbiome interconnectivity. To address this question, we compared bacterial populations identified in matched breast and faecal samples from our NHP study. Dietary pattern concurrently shifted two species in both regions; Streptococcus lutetiensis and Ruminococcus torques. While we observe similar trends in Lactobacillus abundances in the breast and gut, the species identified in each region vary; Mediterranean diet increased Lactobacillus_unspecified species in breast but regulated L. animalis and L. reuteri in the gut.We also investigated the impact of gut permeability on the breast microbiome. Regardless of dietary pattern, subjects that displayed increased physiological measures of gut permeability (elevated plasma lipopolysaccharide, decreased villi length, and decreased goblet cells) displayed a significantly different breast microbiome. Gut barrier dysfunction was associated with increased alpha-diversity and significant different beta-diversity in the breast tissue. Taken together our data supports the presence of a breast microbiome influenced by diet that largely varies from the gut microbiome population but is, however, sensitive to gut permeability.