Permeability of fluorescent probes at phase transitions from bilayer-coated capsule membranes
Permeability of fluorescent probes at phase transitions from bilayer-coated capsule membranes
复制标题
双层涂层胶囊膜相变时荧光探针的渗透性
DOI：
10.1039/p29850001591
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
T. Seki
中科院分区：
文献类型：
--
作者：
Y. Okahata;Naomi Iizuka;G. Nakamura;T. Seki
制备了涂有合成双层的超薄尼龙胶囊膜，制备了载有阳离子，阴离子和zwitterionic荷的亲水性头部基团。使用双层涂层的胶囊膜来测量整个膜的13个水溶性荧光探针的渗透性，具有阳离子，阴离子，ZWITTERICINIC和非离子水生基团。与脂质体膜相比，在胶囊膜上支撑的双层更适合于渗透和捕获实验的动力学研究。荧光探针的渗透性在涂层双层的相过渡温度（TC）附近发生了巨大变化，具体取决于双层和药性探针的亲水基团的电荷。从Arrhenius图的活化能数据和形状中，与小型电解质（如NaCl）相比，讨论了TC下方和上方的大探针分子的渗透过程。
Ultrathin nylon capsule membranes coated with synthetic bilayers, the hydrophilic head groups of which carried cationic, anionic, and zwitterionic charges, were prepared. The bilayer-coated capsule membranes were used to measure the permeability of 13 water-soluble fluorescent probes, having cationic, anionic, zwitterionic, and non-ionic hydrophilic groups, across the membrane. A bilayer supported on a capsule membrane is more suitable for kinetic studies of permeation and trapping experiments than is a liposomal membrane. The permeability of fluorescent probes was drastically changed near a phase-transition temperature (Tc) of a coating bilayer, depending on the charges on the hydrophilic groups of both bilayers and permeant probes. From both activation energy data and shapes of Arrhenius plots, the permeation process of large probe molecules below and above the Tc is discussed, in comparison with that of a small electrolyte such as NaCl.