Two tetratricopeptide repeat proteins facilitate human aryl hydrocarbon receptor signalling in yeast
Two tetratricopeptide repeat proteins facilitate human aryl hydrocarbon receptor signalling in yeast
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DOI：
10.1016/s0898-6568(02)00002-5
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发表时间：
2002-07-01
影响因子：
4.8
通讯作者：
Miller, CA
中科院分区：
文献类型：
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作者：
Miller, CA
在酵母中构建了人芳烃 (Ah) 受体信号通路，并用于鉴定可能与哺乳动物细胞中存在的调节蛋白相关的调节蛋白。人乙型肝炎蛋白 X 相关蛋白 2 (XAP2) 蛋白与酵母 Cpr7 和 Cns1 蛋白的序列相似性表明这些蛋白可能参与该模型系统中的 Ah 受体信号传导。在缺乏 Cpr7 的细胞中，来自 lacZ 报告基因的 Ah 受体信号减少了约 60%。体外相互作用实验表明Cpr7-GST融合蛋白和Ah受体形成复合物。 Cpr7 的表达。 Cns1 和从质粒中分离出的 Cpr7 四肽重复 (TPR) 区域恢复了 Cpr7 缺陷菌株中的 Ah 受体信号传导功能。因此。 Cpr7 和 Cns1 蛋白促进酵母中表达的人类 Ah 受体的信号传导，可能与哺乳动物细胞中含有 TPR 的 XAP2 蛋白和相关伴侣蛋白的方式相同。 (C) 2002 Elsevier Science Inc. 保留所有权利。
A human aryl hydrocarbon (Ah) receptor signalling pathway was constructed in yeast and used to identify regulatory proteins that may be related to those present in mammalian cells. The sequence similarity of human hepatitis B protein X-associated protein 2 (XAP2) protein to yeast Cpr7 and Cns1 proteins suggested that these proteins might be involved in Ah receptor signalling in this model system. Ah receptor signalling from a lacZ reporter gene was reduced by similar to60% in cells that lacked Cpr7. In vitro interaction experiments indicated that a Cpr7-GST fusion protein and Ah receptor formed a complex. Expression of Cpr7. Cns1 and the isolated tetratricopeptide repeat (TPR) region of Cpr7 from plasmids restored Ah receptor signalling function in the Cpr7-deficient strain. Thus. Cpr7 and Cns1 proteins facilitate the signalling of human Ah receptor expressed in yeast, perhaps in the same manner as the TPR-containing XAP2 protein and related chaperone proteins in mammalian cells. (C) 2002 Elsevier Science Inc. All rights reserved.