Low temperature-induced chloroplast relocation mediated by a blue light receptor, phototropin 2, in fern gametophytes
Low temperature-induced chloroplast relocation mediated by a blue light receptor, phototropin 2, in fern gametophytes
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DOI：
10.1007/s10265-008-0165-9
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Wada, Masamitsu
中科院分区：
文献类型：
--
作者：
Kodama, Yutaka;Tsuboi, Hidenori;Wada, Masamitsu
叶绿体对光的响应运动已经知道了100多年。叶绿体向弱光移动，远离强光移动。暗诱导的重新定位，称为暗定位，也已被证明。然而，其他刺激对叶绿体运动的影响还没有得到很好的表征。在这里，我们研究了低温诱导的叶绿体搬迁（称为冷定位）在原叶体细胞的蕨类铁线蕨的配子体，veneris。在25 ℃弱光条件下，原叶体细胞中的叶绿体沿着周壁聚集，但当原叶体随后转移到4 ℃时，叶绿体移向垂周壁。温度从25摄氏度到10摄氏度或更低就足以诱导冷定位，而高强度的光增强了这种反应。在低温下，细胞核也从平周位置（沿着平周壁的位置）重新定位到垂周位置（沿着垂周壁的位置），而线粒体则没有。冷定位没有观察到突变蕨类配子体缺陷的蓝光感光体，光致蛋白2。
Chloroplast movement in response to light has been known more than 100 years. Chloroplasts move towards weak light and move away from strong light. Dark-induced relocation, called dark positioning, has also been shown. However, the effects of other stimuli on chloroplast movement have not been well characterized. Here we studied low temperature-induced chloroplast relocation (termed cold positioning) in prothallial cells of the gametophytes of the fern Adiantum capillus-veneris. Under weak light chloroplasts in prothallial cells accumulated along the periclinal wall at 25 degrees C, but they moved towards anticlinal walls when the prothalli were subsequently transferred to 4 degrees C. A temperature shift from 25 degrees to 10 degrees C or lower was enough to induce cold positioning, and high-intensity light enhanced the response. Nuclei also relocated from the periclinal position (a position along periclinal walls) to the anticlinal position (a position along anticlinal walls) under cold temperature, whereas mitochondria did not. Cold positioning was not observed in mutant fern gametophytes defective of the blue light photoreceptor, phototropin 2.