Spectrally enhanced imaging of occlusal surfaces and artificial shallow enamel erosions with a scanning fiber endoscope

Spectrally enhanced imaging of occlusal surfaces and artificial shallow enamel erosions with a scanning fiber endoscope
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DOI:
10.1117/1.jbo.17.7.076019
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发表时间:
2012-07-01
影响因子:
3.5
通讯作者:
Seibel, Eric J.
Seibel, Eric J.
中科院分区:
医学3区
文献类型:
--
作者:
Zhang, Liang;Nelson, Leonard Y.;Seibel, Eric J.

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超薄扫描纤维内窥镜最初是为癌症诊断而开发的,用于对牙齿咬合表面以及人类拔牙的浅层人工诱导牙釉质侵蚀进行成像(n = 40)。使用短波长 405 nm 照明激光获得了增强的咬合面图像分辨率。此外,还使用 ​​405、404、532 和 635 nm 照明激光对不同深度的人工侵蚀进行成像。还获得了使用 405 nm 照明的激光诱导自发荧光图像。对于每种成像方式,定量计算声音和侵蚀牙釉质之间的对比度。对于浅侵蚀,405 nm 反射图像与完整牙釉质的图像对比度最大。还确定对比度的增加很大程度上是由于体积散射和表面散射的较小成分所致。此外,使用浅穿透深度照明激光(405 nm)获得的图像提供了表面釉质形貌的最详细信息,因为反射光不包含从釉质组织内更深处反射的光的贡献。还进行了多层蒙特卡罗模拟以证实实验结果。 (C) 2012 年光电仪器工程师协会 (SPIE)。偶像10.1117/1.JBO.17.7.076019]
An ultrathin scanning fiber endoscope, originally developed for cancer diagnosis, was used to image dental occlusal surfaces as well as shallow artificially induced enamel erosions from human extracted teeth (n = 40). Enhanced image resolution of occlusal surfaces was obtained using a short-wavelength 405-nm illumination laser. In addition, artificial erosions of varying depths were also imaged with 405-, 404-, 532-, and 635-nm illumination lasers. Laser-induced autofluorescence images of the teeth using 405-nm illumination were also obtained. Contrast between sound and eroded enamel was quantitatively computed for each imaging modality. For shallow erosions, the image contrast with respect to sound enamel was greatest for the 405-nm reflected image. It was also determined that the increased contrast was in large part due to volume scattering with a smaller component from surface scattering. Furthermore, images obtained with a shallow penetration depth illumination laser (405 nm) provided the greatest detail of surface enamel topography since the reflected light does not contain contributions from light reflected from greater depths within the enamel tissue. Multilayered Monte Carlo simulations were also performed to confirm the experimental results. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). IDOL 10.1117/1.JBO.17.7.076019]