The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis
The role of vascular endothelial growth factor and mesenchymal stem cells during angiogenesis
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DOI：
10.4066/biomedicalresearch.29-18-887
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发表时间：
2018
影响因子：
1.2
通讯作者：
Nanae Oki;T. Abe;R. Kunimatsu;Keisuke Sumi;T. Awada;Y. Tsuka;K. Nakajima;K. Ando;K. Tanimoto
中科院分区：
文献类型：
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作者：
Nanae Oki;T. Abe;R. Kunimatsu;Keisuke Sumi;T. Awada;Y. Tsuka;K. Nakajima;K. Ando;K. Tanimoto
目的：血管生成是骨重建和骨再生所必需的。我们以前的研究表明，将间充质干细胞（MSCs）移植到骨缺损区域3个月后，MSC侧的再生骨明显多于对照侧。在移植有MSCs的一侧，血管数量显著增加。因此，在重建过程中，骨髓间充质干细胞，这是必要的再生骨，参与骨再生通过血管生成在移植区。此外，MSC产生血管内皮生长因子（VEGF）。因此，我们研究了MSCs分化对VEGF表达的影响。研究方法：我们研究了MSCs成骨分化的变化如何影响VEGF基因和蛋白表达，以及与人脐静脉内皮细胞（HUVECs）共培养如何影响MSCs中的基因表达。我们还确定了MSC在移植区域中的作用，以及与MSC共培养时对HUVEC形态的影响。结果：未分化MSCs中VEGF基因及蛋白表达均高于分化MSCs。与HUVECs共培养的MSCs中VEGF基因的表达高于单独培养的MSCs。在MSC和HUVECs的共培养中，在培养的第3天观察到管腔样结构，随后观察到几个管腔结构。结论：MSC移植可能通过产生VEGF促进血管生成。
Objective: Angiogenesis is essential for bone remodeling and regeneration. We previously showed that 3 months after transplantation of mesenchymal stem cells (MSCs) into a bone defect area, significantly more regenerated bone was present on the MSC side than on the control side. On the side transplanted with MSCs, the number of blood vessels was significantly higher. Therefore, during the remodeling process, MSCs, which are necessary for regenerating bone, were involved in bone regeneration through angiogenesis in the transplanted area. In addition, MSCs produce Vascular Endothelial Growth Factor (VEGF). Therefore, we investigated the influence of differentiation of MSCs on the expression of VEGF. Methods: We investigated how changes in osteogenic differentiation of MSCs affect VEGF gene and protein expression and how co-culture with Human Umbilical Vein Endothelial Cells (HUVECs) affects gene expression in MSCs. We also determined the role MSCs play in the transplanted area, and the effects on HUVEC morphology when co-cultured with MSCs. Results: VEGF gene and protein expression in undifferentiated MSCs was higher than in differentiated MSCs. VEGF gene expression in MSCs co-cultured with HUVECs was higher than when MSCs were cultured alone. In co-cultures of MSCs and HUVECs, a lumen-like structure was observed on the 3rd day of culture, and then several luminal structures were observed later. Conclusion: MSC transplantation may contribute to angiogenesis via production of VEGF.