PNA and LNA throw light on DNA
PNA and LNA throw light on DNA
复制标题
DOI：
10.1016/s0167-7799(02)00008-2
复制
发表时间：
2003-01-01
影响因子：
17.3
通讯作者：
Demidov, VV
中科院分区：
文献类型：
--
作者：
Demidov, VV
在一些方面，同质（全溶液）核酸杂交测定优于传统使用的异质（溶液到表面）替代方法。前荧光探针在与 DNA 和 RNA 靶标结合时显示出荧光增强或荧光偏振，是实时序列特异性核酸同质检测的范例。多种此类不同结构的 DNA 或 RNA 衍生探针已被开发并具有多种应用。然而，最近该领域的新增内容——锁核酸（LNA）和肽核酸（PNA）——显着增加了荧光探针的潜力，并为其新用途提供了强劲的推动力。
In some aspects, homogeneous (all-in-solution) nucleic acid hybridization assays are superior to the traditionally used heterogeneous (solution-to-surface) alternatives. Profluorescent probes, which reveal fluorescence enhancement or fluorescence polarization upon their binding to DNA and RNA targets, are a paradigm for the real-time sequence-specific homogeneous detection of nucleic acids. A variety of such DNA or RNA-derived probes of different constructs has already been developed with numerous applications. However, the recent additions to the field - locked nucleic acids (LNAs) and peptide nucleic acids (PNAs) - significantly increase the potential of profluorescent probes and provide a robust impulse for their new uses.