Corrigendum: Noncovalent Hydrogel Beads as Microcarriers for Cell Culture.
Corrigendum: Noncovalent Hydrogel Beads as Microcarriers for Cell Culture.
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勘误表：非共价水凝胶珠作为细胞培养的微载体
DOI：
10.1002/anie.201607037
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发表时间：
2016
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Wieduwild R;Krishnan S;Chwalek K;Boden A;Nowak M;Drechsel D;Werner C;Zhang Y
水凝胶微球作为微载体在生物技术中有着广泛的应用前景。然而，由于快速凝胶化速率和/或低稳定性，通过非共价交联形成珠粒以通过避免化学反应实现高细胞相容性仍然具有挑战性。在这里，我们报告了使用非共价交联机制在温和的细胞相容性条件下从肽-聚乙二醇缀合物和寡糖制备均匀的、可调节的和稳健的水凝胶珠。大的蛋白质可以很容易地从珠子中释放出来。进一步的非共价修饰允许用各种化合物进行珠标记和官能化。在标准细胞培养条件下和冷冻珠后，包埋细胞的存活率较高，证明其适用于包封和保存细胞。作为功能系统的水凝胶珠已经通过产生具有嵌入的分泌eGFP的昆虫细胞的蛋白质生产微载体来实现。
Hydrogel beads as microcarriers could have many applications in biotechnology. However, bead formation by noncovalent cross‐linking to achieve high cell compatibility by avoiding chemical reactions remains challenging because of rapid gelation rates and/or low stability. Here we report the preparation of homogeneous, tunable, and robust hydrogel beads from peptide–polyethylene glycol conjugates and oligosaccharides under mild, cell‐compatible conditions using a noncovalent crosslinking mechanism. Large proteins can be released from beads easily. Further noncovalent modification allows for bead labeling and functionalization with various compounds. High survival rates of embedded cells were achieved under standard cell culture conditions and after freezing the beads, demonstrating its suitability for encapsulating and conserving cells. Hydrogel beads as functional system have been realized by generating protein‐producing microcarriers with embedded eGFP‐secreting insect cells.