EFFECTS OF CORTISOL AND THYROXINE ON PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLGLYCEROL SYNTHESIS BY ADULT-RAT LUNG ALVEOLAR TYPE-II CELLS IN PRIMARY CULTURE
EFFECTS OF CORTISOL AND THYROXINE ON PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLGLYCEROL SYNTHESIS BY ADULT-RAT LUNG ALVEOLAR TYPE-II CELLS IN PRIMARY CULTURE
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DOI：
10.1016/0005-2760(80)90037-5
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
VANGOLDE, LMG
中科院分区：
文献类型：
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作者：
POST, M;BATENBURG, JJ;VANGOLDE, LMG
在原代培养的成年大鼠肺II型细胞中研究了皮质醇和甲状腺素对磷脂酰胆碱和磷脂酰甘油形成的影响。加入皮质醇可促进[Me-14 C]胆碱、[1- 14 C]乙酸酯、[1- 14 C]棕榈酸酯、D-[U-14 C]葡萄糖和[1（3）-3H]甘油掺入总磷脂酰胆碱和二饱和磷脂酰胆碱中。皮质醇刺激标记的乙酸酯，棕榈酸酯，葡萄糖和甘油的磷脂酰甘油的形成，但不影响磷脂酰乙醇胺的形成。甲状腺素单独没有显着影响总的和二饱和磷脂酰胆碱，也没有磷脂酰甘油或磷脂酰乙醇胺的形成。暴露的细胞的皮质醇和甲状腺素的组合引起的总和disaturated磷脂酰胆碱标记胆碱，棕榈酸和甘油的合成率增加，并在标记甘油的磷脂酰甘油。这些增加与皮质醇单独带来的增加大致相同。皮质醇和甲状腺素的组合并没有显着影响标记的乙酸和葡萄糖进入磷脂酰胆碱和磷脂酰甘油。显然，糖皮质激素对肺泡II型细胞的直接作用可能在成人肺表面活性物质脂质合成的调节中发挥作用。
The effect of cortisol and thyroxine on the formation of phosphatidylcholines and phosphatidylglycerols was studied in adult rat lung type II cells in primary culture. Addition of cortisol enhanced the incorporation of [Me-14C]choline, [1-14C]acetate, [1-14C]palmitate, D-[U-14C]glucose and [1(3)-3H]glycerol into total and disaturated phosphatidylcholines. Cortisol stimulated the formation of phosphatidylglycerols from labelled acetate, palmitate, glucose and glycerol, but did not affect the formation of phosphatidylethanolamines. Thyroxine alone did not significantly affect the formation of total and disaturated phosphatidylcholines nor that of phosphatidylglycerols or phosphatidylethanolamines. Exposure of the cells to a combination of cortisol and thyroxine caused increases in the rates of synthesis of total and disaturated phosphatidylcholines from labelled choline, palmitate and glycerol and in that of phosphatidylglycerols from labelled glycerol. These increases were about the same as those brought about by cortisol alone. The combination of cortisol and thyroxine did not significantly affect the entry of labelled acetate and glucose into phosphatidylcholines and phosphatidylglycerols. Apparently, direct effects of glucocorticosteroids on the alveolar type II cell may play a role in the regulation of the synthesis of surfactant lipids in the adult lung.