Antibodies from dogs with canine visceral leishmaniasis recognise two proteins from the saliva of Lutzomyia longipalpis
Antibodies from dogs with canine visceral leishmaniasis recognise two proteins from the saliva of Lutzomyia longipalpis
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DOI：
10.1007/s00436-006-0307-8
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发表时间：
2007-02-01
影响因子：
2
通讯作者：
Reis, Alexandre Barbosa
中科院分区：
文献类型：
--
作者：
Bahia, Diana;Gontijo, Nelder Figueiredo;Reis, Alexandre Barbosa
沙蝇的唾液是利什曼原虫的主要媒介，其唾液具有药理和免疫调节活性，可能有助于寄生虫进入和建立脊椎动物宿主。因此，沙蝇的唾液腺成分是开发人类利什曼病疫苗的潜在候选者。为了鉴定可用于免疫动物对抗犬内脏利什曼病的沙蝇唾液蛋白，我们用自然感染利什曼原虫的狗的血清样本评估了抗唾液抗体的反应性。在Western印迹实验中，狗抗体可识别两种相对分子质量为28.6和47.3 kDa的蛋白质。从SDS-PAGE凝胶中切下蛋白质条带，用质谱仪测定其序列。这两种蛋白质分别被鉴定为Lulo-D7和Lulo Huang。在多组分疫苗实验发展的背景下，讨论了这些发现的意义。
The saliva of the sand fly Lutzomyia longipalpis, a major vector of Leishmania, exhibits pharmacological and immunomodulatory activities that may facilitate entry and establishment of parasites into the vertebrate host. Salivary gland components of the sand fly are, therefore, potential candidates in the development of a vaccine against human leishmaniasis. With the objective of identifying sand fly saliva proteins that could be used to immunise animals against canine visceral leishmaniasis, we have evaluated anti-saliva antibody reactivity using serum samples collected from dogs naturally infected with Leishmania chagasi. Two proteins with molecular weights of 28.6 and 47.3 kDa were recognised by dog antibodies in Western blot assays. Protein bands were excised from an SDS-PAGE gel and the sequences determined by mass spectrometry. The proteins were identified as LuLo-D7 and Lulo YELLOW, respectively. The significance of these findings in the context of the development of multi-component vaccination experiments is discussed.