Selective reduction of intestinal trefoil factor in untreated coeliac disease
Selective reduction of intestinal trefoil factor in untreated coeliac disease
复制标题
DOI：
10.1046/j.1365-2249.2002.02011.x
复制
发表时间：
2002-12-01
影响因子：
4.6
通讯作者：
Mahida, YR
中科院分区：
文献类型：
--
作者：
Ciacci, C;Di Vizio, D;Mahida, YR
三叶因子家族(TFF)包括小肽，其中肠道三叶因子(ITF)在小肠和大肠的杯状细胞中特异表达。以往的研究表明，ITF在粘膜保护和修复中发挥着重要作用。乳糜泻代表了一种免疫介导的小肠炎症和损伤模型，通过无麸质饮食恢复。本研究的目的是研究无面筋饮食治疗前后十二指肠远端粘膜中ITF的表达情况。应用免疫组织化学和半定量聚合酶链式反应(PCR)技术，对11例治疗组、9例未治疗组和8例对照组十二指肠远端活检组织中ITF和粘蛋白的表达进行了研究。在未经治疗的乳糜泻患者中，杯状细胞中ITF免疫反应减弱，但粘蛋白表达得以保留。无麸质饮食的粘膜恢复与杯状细胞中ITF免疫反应性的增加有关。在未经处理的十二指肠样本中，ITF转录本的表达相对于MUC2基因的表达也有所减少，而无面筋饮食的患者则有所恢复。我们的研究表明，在未经治疗的腹部疾病中，ITF基因的表达存在选择性降低。在无麸质饮食中ITF表达的恢复表明，粘膜免疫系统调节人类肠道粘膜中杯状细胞的分化和ITF的表达。
The trefoil factor family (TFF) encompasses small peptides of which intestinal trefoil factor (ITF) is expressed specifically in goblet cells of the small and large intestine. Previous studies have shown that ITF plays an important role in mucosal protection and repair. Coeliac disease represents a model of immune-mediated small intestinal inflammation and damage, with recovery on gluten-free diet. The aim of this study was to investigate the expression of ITF in the distal duodenal mucosa of subjects with coeliac disease, before and after treatment with a gluten-free diet. Expression of ITF and mucin in the distal duodenal biopsies from treated (n = 11) and untreated (n = 9) coeliac subjects and controls (n = 8) was investigated by immunohistochemistry and semiquantitative PCR. In untreated coeliac disease, there was reduction of ITF immunoreactivity in goblet cells but mucin expression was preserved. Mucosal recovery on gluten-free diet was associated with increased ITF immunoreactivity in goblet cells. There was also reduction in the expression of ITF transcripts, relative to MUC2 mRNA, in untreated coeliac duodenal samples, with recovery on gluten-free diet. Our study suggests that there is a selective reduction in the expression of the ITF gene in untreated coeliac disease. Recovery of ITF expression on a gluten-free diet suggests that the mucosal immune system regulates goblet cell differentiation and ITF expression in the human intestinal mucosa.