SYNTHESIS OF TAURINE IN RAT-LIVER AND BRAIN INVIVO
SYNTHESIS OF TAURINE IN RAT-LIVER AND BRAIN INVIVO
复制标题
DOI：
10.1007/bf00964232
复制
发表时间：
1980-01-01
影响因子：
4.4
通讯作者：
MANDEL, P
中科院分区：
文献类型：
--
作者：
PASANTESMORALES, H;CHATAGNER, F;MANDEL, P
在脑池内注射[35 S]半胱氨酸和腹腔注射[3 H]半胱氨酸后的不同时间，在大鼠脑和肝脏中检查牛磺酸的体内形成和标记牛磺酸前体的分析。肝脏和脑中的放射性分布模式相似。在这两个器官中，85%的大脑和80%的肝脏标记在谷胱甘肽（氧化和还原）、半胱氨酸、半胱氨酸亚磺酸、亚牛磺酸、胱硫醚和半胱氨酸和谷胱甘肽的混合二硫化物中回收。半胱氨酸亚磺酸和牛磺酸在肝脏和脑中的相对标记率表明，肝脏比脑具有更高的牛磺酸合成能力。腹腔注射[~ 3 H]半胱氨酸后，在脑中检测到少量[~ 3 H]牛磺酸。这种[3 H]牛磺酸的出现时间以及在脑或血浆中检测不到[3 H]半胱氨酸时发生的事实表明，它可能不是在脑中从标记的前体合成的，而是在其他地方形成并通过交换过程转运到脑中。
The in vivo formation of taurine and the analysis of labeled taurine precursors was examined in rat brain and liver at different times after an intracisternal injection of [35S]cysteine and an i.p. injection of [3H]cysteine, simultaneously administered. The distribution pattern of radioactivity was similar in liver and brain. Most of the labeling, in both organs 85% in brain and 80% in liver was recovered in glutathione (oxidized and reduced), cysteic acid, cysteine sulfinic acid, hypotaurine, cystathionine, and a mixed disulfide of cysteine and glutathione. The relative rates of labeling of cysteine sulfinic acid and taurine in liver and brain suggest than in vivo, liver possesses a higher capacity for taurine synthesis than brain. A small amount of [3H]taurine was detected in brain after i.p. injection of [3H]cysteine. The time of appearance of this [3H]taurine as well as the fact that it occurs when [3H]cysteine is not detectable in brain or plasma suggests that it was probably not synthesized in brain from labeled precursors but formed elsewhere and transported into the brain through an exchange process.