Mimicking the mechanical properties of the cell cortex by the self-assembly of an actin cortex in vesicles
Mimicking the mechanical properties of the cell cortex by the self-assembly of an actin cortex in vesicles
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DOI：
10.1063/1.4871861
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发表时间：
2014-04-14
影响因子：
4
通讯作者：
Robinson, Douglas N.
中科院分区：
文献类型：
--
作者：
Luo, Tianzhi;Srivastava, Vasudha;Robinson, Douglas N.
肌动蛋白骨架与质膜的复合在许多生物事件中起着重要的作用。本研究采用乳液法制备了具有仿生肌动蛋白皮质的囊泡人工细胞，并对其力学性能进行了表征。我们证明了乳化方法提供了调节人工细胞中的脂质组成和蛋白质浓度的灵活性，以达到所需的尺寸分布、内部微观结构和机械性能。此外，将重建的人造细胞与真实细胞（包括使用基因耗尽和药物抑制的细胞）的皮质弹性进行比较，有力地支持了肌动蛋白细胞骨架蛋白在皮质力学中比脂质分子占主导地位。我们的研究表明，用纯化的细胞成分在人工细胞中组装生物系统为回答生物学问题提供了一种有力的方法。(C) 2014 AIP出版有限责任公司
The composite of the actin cytoskeleton and plasma membrane plays important roles in many biological events. Here, we employed the emulsion method to synthesize artificial cells with biomimetic actin cortex in vesicles and characterized their mechanical properties. We demonstrated that the emulsion method provides the flexibility to adjust the lipid composition and protein concentrations in artificial cells to achieve the desired size distribution, internal microstructure, and mechanical properties. Moreover, comparison of the cortical elasticity measured for reconstituted artificial cells to that of real cells, including those manipulated using genetic depletion and pharmacological inhibition, strongly supports that actin cytoskeletal proteins are dominant over lipid molecules in cortical mechanics. Our study indicates that the assembly of biological systems in artificial cells with purified cellular components provides a powerful way to answer biological questions. (C) 2014 AIP Publishing LLC.