Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor
Growth phase-dependent changes in the subcellular localization of pre-B-cell colony-enhancing factor
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DOI：
10.1016/s0014-5793(03)00476-9
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发表时间：
2003-06-05
期刊：
影响因子：
3.5
通讯作者：
Fujisawa, H
中科院分区：
文献类型：
--
作者：
Kitani, T;Okuno, S;Fujisawa, H
从大鼠cDNA文库中分离出一个编码人前B细胞集落增强因子（PBEF）同源物的cDNA。该蛋白质存在于细胞的细胞质和细胞核中，并且在增殖的PC-12和Swiss 3 T3细胞中，细胞质中的量高于细胞核中的量，但是在用神经生长因子处理的PC-12细胞和生长至汇合状态的3 T3细胞中，细胞核中的量高于细胞质中的量。因此，所谓的PBEF不是一种类似于腺嘌呤的分泌蛋白，而是一种与细胞周期相关的细胞内蛋白。(C)2003年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
A cDNA encoding the homolog of the human pre-B-cell colony-enhancing factor (PBEF), a cytokine-like secreted protein, was isolated from a rat cDNA library. This protein existed in both the cytoplasm and nucleus of the cells, and the amount was higher in the cytoplasm than in the nucleus of proliferating PC-12 and Swiss 3T3 cells but higher in the nucleus than in the cytoplasm of the PC-12 cells treated with nerve growth factor and the 3T3 cells grown to a confluent state. Thus, the so-called PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.