Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
Variation in rhizosphere microbial communities and its association with the symbiotic efficiency of rhizobia in soybean
复制标题
大豆根际微生物群落变化及其与根瘤菌共生效率的关系
DOI：
10.1038/s41396-020-0648-9
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发表时间：
2020-04-27
期刊：
影响因子：
11
通讯作者：
Li, Xia
中科院分区：
文献类型：
--
作者：
Han, Qin;Ma, Qun;Li, Xia
根瘤菌-豆科植物共生是植物-微生物互惠共生的一种重要类型，但这种共生关系的建立是复杂的，受多种因素的影响。大豆根际具有特定的微生物群落，但这些微生物是否影响根瘤菌的形成还没有得到很好的研究。本文分析了3种土壤中大豆根区微生物区系的组成及其相互关系。首先，我们发现不同土壤中大豆根际细菌群落组成存在显著差异，并对根瘤菌与其他根际细菌之间的关联网络进行了研究。其次，我们发现根瘤中慢生根瘤菌和中华根瘤菌的组成与某些根际微生物有一定的相关性。我们从碱性土壤中培养了278株芽孢杆菌候选菌株。最后，相互作用和结瘤试验表明，蜡状芽孢杆菌组特异性地促进和抑制中华根瘤菌和慢生根瘤菌的生长，分别，并阐明了盐碱条件对中华根瘤菌结瘤的影响，以及影响其在根瘤中的定殖。我们的研究结果证明了细菌微生物群在塑造大豆根瘤菌-宿主相互作用中的关键作用，并提供了一个框架，通过使用合成细菌群落来提高该互利共生系统的共生效率。
Rhizobia-legume symbiosis is an important type of plant-microbe mutualism; however, the establishment of this association is complicated and can be affected by many factors. The soybean rhizosphere has a specific microbial community, yet whether these organisms affect rhizobial nodulation has not been well investigated. Here, we analyzed the compositions and relationships of soybean rhizocompartment microbiota in three types of soil. First, we found that the rhizosphere community composition of soybean varied significantly in different soils, and the association network between rhizobia and other rhizosphere bacteria was examined. Second, we found that some rhizosphere microbes were correlated with the composition of bradyrhizobia and sinorhizobia in nodules. We cultivated 278 candidate Bacillus isolates from alkaline soil. Finally, interaction and nodulation assays showed that the Bacillus cereus group specifically promotes and suppresses the growth of sinorhizobia and bradyrhizobia, respectively, and alleviates the effects of saline-alkali conditions on the nodulation of sinorhizobia as well as affecting its colonization in nodules. Our findings demonstrate a crucial role of the bacterial microbiota in shaping rhizobia-host interactions in soybean, and provide a framework for improving the symbiotic efficiency of this system of mutualism through the use of synthetic bacterial communities.