Enhancement of Phytoalexin Synthesis during Rice Blast Infection of Leaves by Pre-treatment with Carpropamid
Enhancement of Phytoalexin Synthesis during Rice Blast Infection of Leaves by Pre-treatment with Carpropamid
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卡草胺预处理增强稻瘟病叶片中植物抗毒素的合成
DOI：
10.1584/jpestics.24.369
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发表时间：
1999
影响因子：
2.4
通讯作者：
Y. Kurahashi
中科院分区：
文献类型：
--
作者：
Y. Araki;Y. Kurahashi
黑色素生物合成抑制剂Carpropamid {（1 RS，3SR）-2，2-dichloro-N-[1-（4-chlorophenyl）ethyl]-1-ethyl-3-methyl cyclopropanicarboxamide}能促进稻瘟病菌（Pyricularia vulgaris）感染后水稻叶片中植物抗毒素momilactone A和樱花内酯的积累。环丙酰菌胺及其两种非对映异构体A（1 R，3S）和B（1 S，3R）不能直接刺激植物抗毒素的产生，但它们能增强水稻对稻瘟病的反应，使两种植物抗毒素的产生更快、更大量。用WL 28325（2，2-二氯-3，3-二甲基环丙烷羧酸）处理的水稻叶片也观察到这种现象。植物抗毒素积累量与化学物质浓度和生物效应之间的相关性计算数据表明，异构体A的相关模式与WL 28325相似。异构体A的主要作用模式似乎是与WL 28325类似的在稻瘟病感染期间增强植物抗毒素合成（EPAS模式），而不是抑制黑色素生物合成（MBI模式）。相反，异构体B的主要作用模式被认为是MBI，尽管EPAS可能也有助于其生物活性。Carpropamid被认为是通过MBI和EPAS活动的组合来控制稻瘟病。
Carpropamid {(1RS, 3SR)-2,2-dichloro-N-[1-(4-chlorophenyl) ethyl]-1-ethyl-3-methyl cyclo-propanecarboxamide}, which is considered as a melanin biosynthesis inhibitor, enhanced the accumulation of the phytoalexins momilactone A and sakuranetin in the rice leaves subsequently inoculated with the blast pathogen (Pyricularia oryzae). Carpropamid and its two diastereo-isomers, A(1R,3S) and B(1S,3R), did not stimulate phytoalexin production directly, but they potentiated rice plants to produce the two phytoalexins more rapidly and much amounts in response to blast infection. This phenomenon was also observed in rice leaves treated with WL28325 (2,2-dichloro-3,3-dimethylcyclopropane carboxylic acid), a resistance inducer. The calculation data of the correlation among accumulation of phytoalexins, concentrations of chemicals and biological effects suggested that the correlation pattern of isomer A resembled that of WL28325. The main mode of action of isomer A appears to be an enhancement of phytoalexin synthesis during blast infection (EPAS mode) similar to that of WL28325, rather than the inhibition of melanin biosynthesis (MBI mode). In contrast, the main mode of action of isomer B was found to be MBI, although EPAS probably also contributes to its biological activity. Carpropamid is assumed to control rice blast through a combination of MBI and EPAS activities.