Enzymatic hydrolysis of pretreated rice straw
Enzymatic hydrolysis of pretreated rice straw
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DOI：
10.1016/s0960-8524(96)00169-1
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发表时间：
1997-02-01
影响因子：
11.4
通讯作者：
Shoemaker, SP
中科院分区：
文献类型：
--
作者：
Vlasenko, EY;Ding, H;Shoemaker, SP
加州的稻草正在被评估作为生产电力和燃料的原料。本文研究了该工艺的初始步骤：稻草的预处理和预处理材料中的多糖的酶水解为可溶性糖。稻草经过三种不同的预处理程序：酸催化蒸汽爆炸（天鹅生物质公司），酸水解（美国能源部国家可再生能源实验室），和氨纤维爆炸或AFEX（得克萨斯A & M大学）。每种预处理的标准条件，但没有一个是优化的稻草具体。六种商业纤维素酶，Genencor International（USA）、Novo（丹麦）、Iogen（加拿大）和Fermtech（俄罗斯）的产品用于水解。天鹅和酸预处理有效地去除半纤维素从稻草，提供高产量的可发酵糖。AFEX预处理与其他预处理的明显不同之处在于，它没有显着溶解半纤维素。这三种预处理程序都显著提高了稻草的酶消化率。三种商业里氏木霉衍生的酶制剂：纤维素酶IOOL（Iogen）、Spezyme CP（Genencor）和Al（Fermtech）对预处理的稻草比其他测试的酶制剂更有活性。对纤维素酶IOOL水解稻草的条件进行了研究。纤维二糖酶（Novozyme 188）的这种酶制剂的补充显着改善天鹅和酸预处理的材料的水解参数，但不影响AFEX预处理的稻草的水解。根据酶解释放的可发酵碳水化合物的总产量和分布，比较了三种预处理技术（使用最高可能的底物浓度，Swan-和酸-为150 g/l，AFEX-预处理的秸秆为100 g/l，酶负载量为6.7滤纸单位（FPU）和6.7纤维二糖酶单位（CBU）对于所有预处理的材料来说，每克干稻草的量是相同的）。通过预处理步骤和通过酶促水解产生的单糖的合并产率被发现分别为46、42和37 g/l，用于天鹅、酸和AFEX预处理的稻草。(C)爱思唯尔科技有限公司
California rice straw is being evaluated as a feedstock for production of power and fuel. This paper examines the initial steps in the process: pretreatment of rice straw and enzymatic hydrolysis of the polysaccharides in the pretreated material to soluble sugars. Rice straw was subjected to three distinct pretreatment procedures: acid-catalyzed steam explosion (Swan Biomass Company), acid hydrolysis (U.S. DOE National Renewable Energy Laboratory), and ammonia fiber explosion or AFEX (Texas A & M University). Standard conditions for each pretreatment were used but none was optimized for rice straw specifically. Six commercial cellulases, products of Genencor International (USA), Novo (Denmark), Iogen (Canada) and Fermtech (Russia) were used for hydrolysis. The Swan- and the acid-pretreatments effectively removed hemicellulose from rice straw, providing high yields of fermentable sugars. The AFEX-pretreatment was distinctly different from other pretreatments in that it did not significantly solubilize hemicellulose. All three pretreatment procedures substantially increased enzymatic digestibility of rice straw. Three commercial Trichoderma-reesei-derived enzyme preparations: Cellulase IOOL (Iogen), Spezyme CP (Genencor), and Al (Fermtech), were more active on pretreated rice straw compared than others tested. Conditions for hydrolysis of rice straw using Cellulase IOOL were evaluated. The supplementation of this enzyme preparation with cellobiase (Novozyme 188) significantly improved the parameters of hydrolysis for the Swan- and the acid-pretreated materials, but did not affect the hydrolysis of the AFEX-pretreated rice straw. The three pretreatment techniques were compared on a basis of a total yield and distribution of fermentable carbohydrates released by enzymatic hydrolysis (the highest possible substrate concentrations were used, 150 g/l for the Swan- and the acid- and 100 g/l for the AFEX-pretreated straw enzyme loading of 6.7 filter paper units (FPU) and 6.7 cellobiase units (CBU) per gram of dry straw was the same for all pretreated materials). A combined yield of monosaccharides produced by a pretreatment step and by enzymatic hydrolysis was found to be 46, 42 and 37 g/l for the Swan-, the acid- and the AFEX-pretreated rice straw, respectively. (C) Elsevier Science Ltd.