Structure of toroidal DNA collapsed inside the phage capsid
Structure of toroidal DNA collapsed inside the phage capsid
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DOI：
10.1073/pnas.0901240106
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发表时间：
2009-06-09
影响因子：
11.1
通讯作者：
Livolant, Francoise
中科院分区：
文献类型：
--
作者：
Leforestier, Amelie;Livolant, Francoise
通过使用部分填充的噬菌体衣壳结合冷冻电子显微镜，研究了由不同长度（3，000至55，000 bp）的单个DNA分子组成的DNA环形结构。在选择产生六边形包装的条件下，将四价阳离子精胺扩散穿过衣壳以浓缩DNA。我们的研究结果表明，手性和六方堆积之间产生的挫折导致扭曲壁的形成;螺旋之间的相关性结合其强曲率施加的DNA螺距的变化。
The structure of DNA toroids made of individual DNA molecules of various lengths (3,000 to 55,000 bp) was studied, by using partially filled bacteriophage capsids in conjunction with cryoelectron microscopy. The tetravalent cation spermine was diffused through the capsid to condense the DNA under conditions that were chosen to produce a hexagonal packing. Our results demonstrate that the frustration arising between chirality and hexagonal packing leads to the formation of twist walls; the correlation between helices combined with their strong curvature impose variations of the DNA helical pitch.