Regulation of protein synthesis by estradiol 17 beta, dexamethasone and insulin in primary cultured Xenopus hepatocytes.
Regulation of protein synthesis by estradiol 17 beta, dexamethasone and insulin in primary cultured Xenopus hepatocytes.
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原代培养的非洲爪蟾肝细胞中雌二醇 17β、地塞米松和胰岛素对蛋白质合成的调节。
DOI：
10.1016/0014-4827(83)90164-7
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发表时间：
1983
影响因子：
3.7
通讯作者：
M. Amano
中科院分区：
文献类型：
--
作者：
A. Kawahara;K. Sato;M. Amano
用无血清原代培养的肝细胞研究了胰岛素、雌二醇（E2）和地塞米松对肝细胞蛋白质合成的影响。所有这些激素刺激分泌和细胞内蛋白质的合成。地塞米松诱导或刺激许多蛋白质的合成（虽然数量有限），而雌二醇诱导或刺激相对较少的蛋白质，包括蛋黄前体蛋白卵黄蛋白原。这些蛋白质中的大多数在分子量和/或等电点上不同。当用两种类固醇处理肝细胞时，大多数蛋白质以预期的速率合成，这些速率来自各自类固醇的单次处理。因此，每种类固醇都选择性地刺激其特定蛋白质的合成。然而，观察到异常的蛋白质，其合成仅通过双重处理来刺激。相反，胰岛素似乎导致个体分泌蛋白合成的总体增加。
Changes in the protein synthesis ofXenopushepatocytes caused by insulin, estradiol-17β (estradiol) and dexamethasone were studied by using a primary culture in serum-free medium. All of these hormones stimulated the synthesis of secretory and intracellular proteins. Dexamethasone induced or stimulated the synthesis of many proteins (though limited in number), whereas estradiol induced or stimulated relatively few proteins, including the yolk precursor protein vitellogenin. The majority of these proteins differed in molecular weight and/or isoelectric point. When hepatocytes were treated with both steroids, most of the proteins were synthesized at the rates expected from the single treatment of the respective steroids. Thus, each steroid selectively stimulated the synthesis of its specific proteins. However, exceptional proteins were observed, whose syntheses were stimulated only by double treatment. In contrast, insulin seemed to cause an overall increase in individual secretory protein synthesis.