Postprandial enrichment of remnant lipoproteins with ApoC-I in healthy normolipidemic men with early asymptomatic atherosclerosis
Postprandial enrichment of remnant lipoproteins with ApoC-I in healthy normolipidemic men with early asymptomatic atherosclerosis
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DOI：
10.1161/01.atv.0000029972.42487.42
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发表时间：
2002-09-01
影响因子：
8.7
通讯作者：
Hamsten, A
中科院分区：
文献类型：
--
作者：
Björkegren, J;Silveira, A;Hamsten, A
目的：最近，我们报道了血脂正常的冠心病患者餐后高脂血症与载脂蛋白C-I（apoC-I）富集残余脂蛋白有关。在这项研究中，乳糜微粒残留物和极低密度脂蛋白（VLDL）的数量和组成进行了检查，在30个无症状normolipidemic 50岁的男性，并没有早期颈动脉粥样硬化lesions.Results和方法内膜中层厚度的远壁的颈总动脉B型超声。富含甘油三酯的脂蛋白通过密度梯度超离心进行亚分级，并通过免疫亲和层析分离成VLDL和乳糜微粒残余部分。在患有早期动脉粥样硬化的男性中，餐后甘油三酯血症和富含甘油三酯的脂蛋白颗粒数量（即载脂蛋白B浓度）的增加并没有被夸大。相反，它们的大（Svedberg浮选率60至400）和小（Svedberg浮选率20至60）乳糜微粒残留物和VLDL极大地富集了apoC-I，它们的小乳糜微粒残留物和VLDL颗粒相对富集了胆固醇。此外，小乳糜微粒残余物上的apoC-I分子的数量与atherosclerosis. Conclusions的程度密切相关，早期无症状动脉粥样硬化在normolipidemic男性没有夸张的餐后高脂血症与餐后乳糜微粒和VLDL颗粒与apoC-I富集。因此，可以想象脂蛋白残余物中的apoC-I含量可以作为冠状动脉疾病风险的早期标志物。
Objectives-Recently, we reported that exaggerated postprandial triglyceridemia in normolipidemic patients with coronary artery disease is associated with enrichment of remnant lipoproteins with apolipoprotein C-I (apoC-I). In this study, the number and composition of chylomicron remnants and very low density lipoproteins (VLDLs) were examined in 30 asymptomatic normolipidemic 50-year-old men with and without early carotid atherosclerotic lesions.Results and Methods-Intima-media thickness of the far wall of the common carotid artery was determined by B-mode ultrasound. Triglyceride-rich lipoproteins were subfractionated by density gradient ultracentrifugation and separated into VLDL and chylomicron remnant fractions by immunoaffinity chromatography. The postprandial triglyceridemia and increase in triglyceride-rich lipoprotein particle number (ie, apolipoprotein B concentrations) were not exaggerated in men with early atherosclerosis. In contrast, their large (Svedberg flotation rate 60 to 400) and small (Svedberg flotation rate 20 to 60) chylomicron remnants and VLDL were greatly enriched with apoC-I, and their small chylomicron remnants and VLDL particles were relatively enriched with cholesterol. Moreover, the number of apoC-I molecules on small chylomicron remnants was strongly associated with the degree of atherosclerosis.Conclusions-Early asymptomatic atherosclerosis in normolipidemic men without exaggerated postprandial triglyceridemia is associated with the enrichment of postprandial chylomicron and VLDL particles with apoC-I. Therefore, it is conceivable that the apoC-I content of lipoprotein remnants may serve as an early marker of coronary artery disease risk.