Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force
Intravital Microscopy of Localized Stem Cell Delivery Using Microbubbles and Acoustic Radiation Force
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DOI：
10.1002/bit.25337
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发表时间：
2015-01-01
影响因子：
3.8
通讯作者：
de Jong, N.
中科院分区：
文献类型：
--
作者：
Kokhuis, T. J. A.;Skachkov, I.;de Jong, N.
使用干细胞修复心肌梗死后受损的心脏组织有很大的希望。然而，实验研究中的一个常见发现是在风险区域递送的细胞数量较少。为了改善递送，我们目前正在研究一种新的递送平台，其中干细胞与靶向微泡结合，产生称为StemBells的回声复合物。这些StemBell响应于传入的超声波而振动，使它们容易受到声辐射力的影响。然后可以使用声力将循环的StemBell从血管的中心线推进到血管壁，促进局部干细胞递送。在这项研究中，我们调查的可行性，操纵StemBells在体内声学注射后，使用鸡胚胎模型。裸干细胞或不饱和干细胞（30个气泡/细胞）可以被推向血管壁并在血管壁处被阻止。在P_ = 200和450 kPa时，测得的平均平动速度分别为61和177 μ m/s。因此，该技术提供了增强和良好控制的干细胞递送的潜力，用于改善心肌梗死后的心脏修复。(C)2014 Wiley Periodicals，Inc.
The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is the low number of cells delivered at the area at risk. To improve the delivery, we are currently investigating a novel delivery platform in which stem cells are conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells. These StemBells vibrate in response to incoming ultrasound waves making them susceptible to acoustic radiation force. The acoustic force can then be employed to propel circulating StemBells from the centerline of the vessel to the wall, facilitating localized stem cell delivery. In this study, we investigate the feasibility of manipulating StemBells acoustically in vivo after injection using a chicken embryo model. Bare stem cells or unsaturated stem cells (30 bubbles/cell) can be propelled toward and arrested at the vessel wall. The mean translational velocities measured are 61 and 177 mu m/s for P_ = 200 and 450 kPa, respectively. This technique therefore offers potential for enhanced and well-controlled stem cell delivery for improved cardiac repair after a myocardial infarction. (C) 2014 Wiley Periodicals, Inc.