COMPARISON OF LOW-PASSAGE AND HIGH-PASSAGE BOVINE TURBINATE CELLS FOR ASSAY OF BOVINE VIRAL DIARRHEA VIRUS
COMPARISON OF LOW-PASSAGE AND HIGH-PASSAGE BOVINE TURBINATE CELLS FOR ASSAY OF BOVINE VIRAL DIARRHEA VIRUS
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DOI：
10.1007/bf01249692
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
SMITH, RL
中科院分区：
文献类型：
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作者：
MCCLURKIN, AW;PIRTLE, EC;SMITH, RL
本发明描述了牛鼻甲细胞培养的制备和维护。该细胞系无牛病毒性腹泻、副流感-3和牛传染性鼻气管炎病毒；虽然在第9代和第81代之间，模式染色体数量发生了显著变化，但没有观察到明确的形态变化。低传代和高传代牛鼻甲细胞对牛病毒性腹泻病毒同样敏感。
The preparation and maintenance of a culture of bovine turbinate cells is described. The cell line is free of bovine viral diarrhea, parainfluenza-3, and infectious bovine rhinotracheitis viruses; and although the modal chromosome number changed significantly between the 9th passage and the 81st passage, no definite morphological changes were observed. Low- and high-passage bovine turbinate cells were equally susceptible to bovine viral diarrhea virus.