Milk casein-based diet containing TGF-beta controls the inflammatory reaction in the HLA-B27 transgenic rat model.
Milk casein-based diet containing TGF-beta controls the inflammatory reaction in the HLA-B27 transgenic rat model.
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DOI：
10.1177/01486071050290s4s141
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发表时间：
2005-07
期刊：
影响因子：
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通讯作者：
E. Schiffrin;M. El Yousfi;M. Faure;Lydia Combaret;A. Donnet;S. Blum;C. Obled;D. Breuillé
中科院分区：
文献类型：
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作者：
E. Schiffrin;M. El Yousfi;M. Faure;Lydia Combaret;A. Donnet;S. Blum;C. Obled;D. Breuillé
背景：一种含有tgf - β的酪蛋白基础配方已成功用于克罗恩病急性发作的青少年。这种分子所起的作用有待证实。我们研究了含有tgf - β的饮食控制HLA-B27转基因大鼠肠道炎症的能力，并将其与不含tgf - β的类似饮食的效果进行了比较。方法对三组大鼠进行实验研究。将HLA-B27/hbeta2M转基因大鼠喂食含有tgf - β的酪蛋白大鼠适应饮食或不含tgf - β的对照酪蛋白饮食。费舍尔控制的动物喂食后者。分析体重、饮食摄入量、组织重量、粪便样本、白细胞计数和急性期反应。通过组织学、髓过氧化物酶和细胞因子mRNA表达来评估肠道炎症。免疫组织化学检测MUC2蛋白表达。检测肌肉蛋白的分解。结果试验饲粮改善了腹泻，增加了粪便干物质和结肠炎症，表现为炎症评分降低（2.43 +/- 1.13 vs 4.42 +/- 0.53, p < 0.05），粘膜厚度降低（431.25 +/- 72.29 vs 508.57 +/- 81.32微米，p = 0.08）， IFNgamma mRNA表达降低。与对照组相比，饲喂tgf - β饲料的HLA大鼠MUC2蛋白表达增加，但未见恢复正常模式。试验饮食还能降低白细胞计数和急性期反应，改善肌肉分解代谢反应。结论含tgf - β饮食对肠道炎症动物模型有良好的保护作用。我们的观察结果支持饮食tgf - β在恢复免疫稳态中的潜在作用。
BACKGROUND A casein-based formula containing TGF-beta has been successfully used in adolescents during acute episodes of Crohn's disease. The role played by this molecule requires confirmation. We have examined the capacity of a TGF-beta containing diet to control the intestinal inflammation in HLA-B27 transgenic rats, and compared its effects with a similar diet devoid of TGF-beta. METHODS Three groups of rats were studied. HLA-B27/hbeta2M transgenic rats were fed with a casein-based rat-adapted diet containing TGF-beta or a control casein-based diet without TGF-beta. Fischer control animals were fed the latter. Body weight, dietary intake, tissue weights, fecal samples, leukocyte counts, and acute phase response were analyzed. Intestinal inflammation was assessed by histology, myeloperoxidase, and mRNA expression of cytokines. MUC2 protein expression was assessed by immunohistochemistry. Breakdown of muscle protein was examined. RESULTS The test diet improved diarrhea increasing the fecal dry matter and the colonic inflammation as shown by a lower inflammatory score (2.43 +/- 1.13 vs 4.42 +/- 0.53, p < .05), lower mucosal thickness (431.25 +/- 72.29 vs 508.57 +/- 81.32 microm, p = .08) and decreased IFNgamma mRNA expression. MUC2 protein expression was increased in HLA rats fed the TGF-beta diet compared with HLA rats fed the control diet, but restitution to normal pattern was not observed. The test diet also decreased leukocytosis and the acute phase response and improved the muscle catabolic response. CONCLUSION The TGF-beta containing diet has a beneficial effect in an animal model of intestinal inflammation. Our observations support a potential role for dietary TGF-beta in the restoration of immune homeostasis.