Antimicrobial Activity and Chemical Composition of Three Essential Oils Extracted from Mediterranean Aromatic Plants
Antimicrobial Activity and Chemical Composition of Three Essential Oils Extracted from Mediterranean Aromatic Plants
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DOI：
10.1089/jmf.2016.0066
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发表时间：
2016-11-01
影响因子：
2.4
通讯作者：
Camele, Ippolito
中科院分区：
文献类型：
--
作者：
Elshafie, Hazem S.;Sakr, Shimaa;Camele, Ippolito
人们对精油（EO）作为传统化学农药的可能替代品越来越感兴趣。采用气相色谱（GC）和气相色谱-质谱（GC-MS）联用技术，对马鞭草、马约拉纳和鼠尾草中提取的3种EO的化学成分进行了表征，并在体外评价了它们对某些植物或人体病原菌的有效性。以大谱杀菌剂嘧菌酯为对照，研究了该化合物对黄瓜炭疽病菌和番茄灰霉病菌的抑菌活性。针对巨大芽孢杆菌、莫哈韦芽孢杆菌和密歇根棒杆菌（G+ve）以及大肠杆菌、野油菜黄单胞菌、萨瓦斯坦假单胞菌和P.与合成的抗生素四环素相比，本发明提供了一种用于治疗菜豆内生真菌（G-ve）的方法。采用96孔微孔板法评价了对上述供试真菌的最低抑菌浓度。结果表明，所研究的三种挥发油的化学结构主要由单萜化合物组成，所有的油都属于化学型香芹酚/百里酚。GC分析结果鉴定了64种化合物，根据它们的质荷比鉴定了它们。此外，不同浓度的研究EO抑制测试微生物的生长以剂量依赖性的方式。
There is a growing interest in essential oils (EOs) as possible alternatives for traditional chemical pesticides. This study was carried out to characterize the chemical composition of the three EOs extracted from Verbena officinalis, Majorana hortensis, and Salvia officinalis using gas chromatography (GC) and GC-mass spectrometry (GC-MS) and to evaluate in vitro their efficacy against some phyto or human pathogens. The antifungal activity was investigated against Colletotrichum acutatum and Botrytis cinerea in comparison with Azoxystrobin as a large spectrum fungicide. Antibacterial activity was evaluated against Bacillus megaterium, Bacillus mojavensis, and Clavibacter michiganensis (G+ve) and Escherichia coli, Xanthomonas campestris, Pseudomonas savastanoi, and P. syringae pv. phaseolicola (G-ve) compared to a synthetic antibiotic tetracycline. Minimum inhibitory concentration was evaluated against the above tested fungi using 96-well microplate method. Results showed that the chemical structure of the three studied EOs was mainly composed of monoterpene compounds and all oils belong to the chemotype carvacrol/thymol. Results of GC analysis identified 64 compounds, which were identified based on their mass to charge ratio. Furthermore, the different concentrations of studied EOs inhibited the growth of tested microorganism in a dose-dependent manner.