Histochemical method for detecting nitric oxide synthase activity in cell cultures
Histochemical method for detecting nitric oxide synthase activity in cell cultures
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DOI：
10.3109/10520299709082208
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发表时间：
1997-01-01
影响因子：
1.6
通讯作者：
Moochhala, SM
中科院分区：
文献类型：
--
作者：
Hon, WM;Chhatwal, VJS;Moochhala, SM
NADPH脱氢酶组织化学已被广泛用于检测各种细胞类型（包括神经细胞体、血管内皮、免疫系统细胞和上皮细胞）中一氧化氮合酶（NOS）的活性，但在细胞培养中使用脱氢酶技术研究NOS的诱导尚未得到研究。在这篇论文中，我们报道了使用diaphorase组织化学作为检测培养细胞中NOS活性的良好标记，该技术可以与其他已建立的技术结合使用，以确定培养细胞中NOS的存在和活性。
NADPH diaphorase histochemistry has been used extensively for detecting nitric oxide synthase (NOS) activity in various cell types including neuronal cell bodies, vascular endothelium, cells of the immune system and epithelial cells, The use of the diaphorase technique in cell cultures to study the induction of NOS has not been investigated. In this paper we report the use of diaphorase histochemistry as a good marker for the detection of NOS activity in cultured cells, This technique can be used in conjunction with other established techniques to determine the presence and activity of NOS in cultured cells.