Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run
Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run
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DOI：
10.1007/s00421-011-1839-x
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发表时间：
2011-09-01
影响因子：
3
通讯作者：
Florida-James, Geraint
中科院分区：
文献类型：
--
作者：
Gomes, Elisa Couto;Stone, Vicki;Florida-James, Geraint
探讨高温高湿臭氧污染环境对8 km计时跑运动员肺部炎症和氧化应激的急性影响。采用单盲随机设计，10名男性运动员（平均值（V）超过dotO（2 max）= 64.4 mlO（2）kg（-1）min（-1），SD = 4.4）在四种不同的环境条件下参加计时赛：20 ℃ + 50%相对湿度（rh）（对照）; 20 ℃ +50%rh + 0.10 ppm O（3）（对照+ O（3））; 31 ℃ +70%rh（加热）; 31 ℃ +70%rh + 0.10 ppm O（3）（加热+ O（3））。在运动后收集血液样本和鼻灌洗物，并分析炎症、上皮损伤和氧化应激标记物。使用重复测量ANOVA和Tukey事后检验分析数据。与对照组相比，Heat + O（3）试验中8 km跑后上呼吸道CC 16浓度（P < 0.05）和GSH/蛋白浓度（P < 0.05）显著升高。试验间中性粒细胞计数无差异。对于所分析的其他抗氧化剂，未观察到差异。高温、高湿和臭氧污染的环境（0.1ppm）在运动员进行8 km计时跑后立即诱发上呼吸道上皮细胞的早期损伤和抗氧化保护过程。
To investigate the acute effect of a hot, humid and ozone-polluted (O(3)) environment on lung inflammation and oxidative tress of runners performing 8 km time trial run. Using a single-blinded randomized design, 10 male athletes (mean (V) over dotO(2max) = 64.4 mlO(2) kg(-1) min(-1), SD = 4.4) took part in a time trial run in four different environmental conditions: 20 degrees C + 50% relative humidity (rh) (Control); 20 degrees C + 50% rh + 0.10 ppm O(3) (Control + O(3)); 31 degrees C + 70% rh (Heat); 31 degrees C + 70% rh + 0.10 ppm O(3) (Heat + O(3)). Blood samples and nasal lavage were collected post-exercise and analyzed for inflammatory, epithelial damage and oxidative stress markers. Data were analyzed using repeated measures ANOVA with Tukey's post hoc test. A significant increase in CC16 concentration (P < 0.05) and GSH/protein concentration (P < 0.05) in the upper respiratory airways was observed following the 8 km run in the Heat + O(3) trial compared with the control trial. There were no differences in the neutrophil counts between trials. No differences were observed for the other antioxidants analyzed. A hot, humid and ozone-polluted environment (0.1 ppm) elicits an early epithelial damage and antioxidant protection process in the upper respiratory airways of athletes immediately after performing 8 km time trial run.