Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger protein matotopetli
Regulation of transcription of meiotic cell cycle and terminal differentiation genes by the testis-specific Zn-finger protein matotopetli
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DOI：
10.1242/dev.01032
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发表时间：
2004-04-01
期刊：
影响因子：
4.6
通讯作者：
White-Cooper, H
中科院分区：
文献类型：
--
作者：
Perezgasga, L;Jiang, JQ;White-Cooper, H
一个强大的发育调节和细胞类型特异性转录程序被激活的初级精母细胞在精子发生过程中的雄性配子的分化准备。在果蝇中的工作开始揭示调节这种基因表达的遗传网络。果蝇aly-class减数分裂阻滞基因座对于许多分化特异性基因的转录激活以及对于减数分裂细胞周期进程重要的几个基因是必不可少的，从而将减数分裂细胞周期进程与精子发生期间的细胞分化联系起来。三个先前描述的aly-class蛋白（aly，comr和achi/维斯）形成一个复合物，并与初级精母细胞中的染色质相关。我们确定，克隆和表征一个新的aly-class减数分裂阻滞基因，matotopetli（topi），它编码一个睾丸特异性锌指蛋白，物理相互作用与Comr。topi突变体表型最像achi/维斯，因为topi功能不是Aly或Comr的核定位所需的，而是它们在染色质上积累所需的。转录程序中的大多数靶基因都依赖于topi和achi/维斯;然而，一小部分靶基因对topi或achi/维斯的丢失具有差异敏感性，这表明这些aly-class预测的DNA结合蛋白在某些情况下可以独立起作用。
A robust developmentally regulated and cell type specific transcriptional programme is activated in primary spermatocytes in preparation for differentiation of the male gametes during spermatogenesis. Work in Drosophila is beginning to reveal the genetic networks that regulate this gene expression. The Drosophila aly-class meiotic arrest loci are essential for activation of transcription of many differentiation-specific genes, as well as several genes important for meiotic cell cycle progression, thus linking meiotic cell cycle progression to cellular differentiation during spermatogenesis. The three previously described aly-class proteins (aly, comr and achi/vis) form a complex and are associated with chromatin in primary spermatocytes. We identify, clone and characterize a new aly-class meiotic arrest gene, matotopetli (topi), which encodes a testis-specific Zn-finger protein that physically interacts with Comr. The topi mutant phenotype is most like achi/vis in that topi function is not required for the nuclear localization of Aly or Comr, but is required for their accumulation on chromatin. Most target genes in the transcriptional programme depend on both topi and achi/vis; however, a small subset of target genes are differentially sensitive to loss of topi or achi/vis, suggesting that these aly-class predicted DNA binding proteins can act independently in some contexts.