Synthesis and properties of uniquely modified oligoribonucleotides: yeast tRNA(Phe) fragments with 6-methyluridine and 5,6-dimethyluridine at site-specific positions.
Synthesis and properties of uniquely modified oligoribonucleotides: yeast tRNA(Phe) fragments with 6-methyluridine and 5,6-dimethyluridine at site-specific positions.
复制标题
独特修饰的寡核糖核苷酸的合成和特性：在特定位点具有 6-甲基尿苷和 5,6-二甲基尿苷的酵母 tRNA(Phe) 片段。
DOI：
10.1080/15257770008035004
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Malkiewicz,A
中科院分区：
文献类型：
--
作者：
Sochacka,E;Czerwinska,G;Guenther,R;Cain,R;Agris,PF;Malkiewicz,A
合成了6-甲基尿苷和5，6-二甲基尿苷的亚磷酰胺，并将修饰的尿苷位点选择性地掺入到与酵母tRNAP反密码子和TΦC结构域序列对应的十七聚体中。通过NMR、MALDI-TOF MS和UV监测的热变性对寡核糖核苷酸进行了表征。与未修饰的RNA相比，6-甲基化的尿苷在聚合物水平上保持了同构型，并且在每个序列位置上使RNA不稳定。RNA双链体稳定性的降低是可预测的。然而，当修饰的尿苷处于环中时，稳定性的丧失是序列上下文依赖性的，并且此时不能从环中的位置预测。
The phosphoramidites of 6-methyluridine and 5,6-dimethyluridine were synthesized and the modified uridines site-selectively incorporated into heptadecamers corresponding in sequence to the yeast tRNAPheanticodon and TΦC domains. The oligoribonucleotides were characterized by NMR, MALDI-TOF MS and UV-monitored thermal denaturations. The 6-methylated uridines retained thesynconformation at the polymer level and in each sequence location destabilized the RNAs compared to that of the unmodified RNA. The decrease in RNA duplex stability is predictable. However, loss of stability when the modified uridine is in a loop is sequence context dependent, and can not, at this time, be predicted from the location in the loop.