Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.
Identification of Cis-Regulatory Modules that Function in the Male Germline of Flowering Plants.
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DOI：
10.1007/978-1-4939-7286-9_22
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发表时间：
2017
影响因子：
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通讯作者：
Benjamin Peters;Jack Aidley;M. Cadzow;D. Twell;L. Brownfield
中科院分区：
文献类型：
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作者：
Benjamin Peters;Jack Aidley;M. Cadzow;D. Twell;L. Brownfield
开花植物的雄性生殖系在雄配子体的营养细胞内发育，并显示出独特的转录谱。理解这种独特细胞谱系发育的关键是确定基因表达在生殖细胞内是如何调节的。这些知识影响了我们对细胞特化、分化和植物育性的理解。在这里，我们描述的方法来确定顺式调控模块（CRM），作为关键的调控区域的启动子生殖系表达的基因。我们详细介绍了系统发育足迹和使用荧光报告花粉的识别和验证标准物质的互补技术。
The male germline of flowering plants develops within the vegetative cell of the male gametophyte and displays a distinct transcriptional profile. Key to understanding the development of this unique cell lineage is determining how gene expression is regulated within germline cells. This knowledge impacts upon our understanding of cell specification, differentiation, and plant fertility. Here, we describe methods to identifycis-regulatory modules (CRMs) that act as key regulatory regions in the promoters of germline-expressed genes. We detail the complimentary techniques of phylogenetic footprinting and the use of fluorescent reporters in pollen for the identification and verification of CRMs.