tyk2, prototype of a novel class of non-receptor tyrosine kinase genes.
tyk2, prototype of a novel class of non-receptor tyrosine kinase genes.
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发表时间：
1990-09
期刊：
影响因子：
8
通讯作者：
I. Firmbach-Kraft;M. Byers;Shows Tb;R. Dalla-Favera;John J. Krolewski
中科院分区：
文献类型：
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作者：
I. Firmbach-Kraft;M. Byers;Shows Tb;R. Dalla-Favera;John J. Krolewski
我们之前通过在低严格杂交条件下用酪氨酸激酶结构域特异性 c-fms 限制性片段筛选人淋巴 cDNA 文库，鉴定了一种新型蛋白酪氨酸激酶基因 tyk2。我们现在已经分离并测序了全长 tyk2 cDNA 克隆；证明该基因在造血和非造血细胞系中广泛表达；并将其映射到染色体 19p13.2。该 cDNA 克隆长 4176 个核苷酸，编码分子量为 134 千道尔顿的推定蛋白质。我们的序列的疏水性分析没有识别出跨膜结构域，而跨膜结构域存在于蛋白酪氨酸激酶受体类的所有成员中；我们也无法检测到在所有先前鉴定的非受体蛋白激酶中发现的 SH2 结构域。因此，我们提出 tyk2 是一类新型非受体蛋白酪氨酸激酶的原型。
We previously identified a novel protein tyrosine kinase gene, tyk2, by screening a human lymphoid cDNA library with a tyrosine kinase domain specific c-fms restriction fragment under low stringency hybridization conditions. We have now isolated and sequenced a full length tyk2 cDNA clone; demonstrated that this gene is widely expressed in hematopoietic and non-hematopoietic cell lines; and mapped it to chromosome 19p13.2. The cDNA clone is 4176 nucleotides long and codes for a putative protein with a molecular weight of 134 kilodaltons. Hydrophobicity analysis of our sequence does not identify a transmembrane domain, which is found in all members of the receptor class of protein tyrosine kinases; nor can we detect an SH2 domain, found in all previously identified non-receptor protein kinases. We therefore propose that tyk2 is the prototype of a new class of non-receptor protein tyrosine kinases.