Cyclooxygenase isoenzyme localization and mRNA expression in rat lungs
Cyclooxygenase isoenzyme localization and mRNA expression in rat lungs
复制标题
大鼠肺中环氧合酶同工酶定位和 mRNA 表达
DOI：
10.1165/ajrcmb.18.4.2939
复制
发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Seeger, W
中科院分区：
文献类型：
--
作者：
Ermert, L;Ermert, M;Seeger, W
前列腺素类的生成可以通过环氧合酶的两种亚型考克斯-1和考克斯-2进行。考克斯-1被认为是普遍表达的，而考克斯-2大多被认为是动态调节的，响应于炎症刺激。考克斯-1和考克斯-2在肺（具有高环氧合酶活性的器官）中的细胞定位尚不清楚。应用免疫金银染色和RT-PCR技术检测正常大鼠肺组织中考克斯-1和考克斯-2的表达和定位。通过测量数字化核外极化图像的平均灰度值对染色强度进行定量图像分析。考克斯-1和考克斯-2的表达在大鼠肺中是容易检测的。考克斯-1免疫反应主要定位于支气管上皮细胞，大门静脉平滑肌细胞，肺泡巨噬细胞和肺动脉内皮细胞（表达较低）。最强的考克斯-2染色见于巨噬细胞和肥大细胞样细胞，在支气管上皮附近和血管周围的结缔组织中检测到。此外，强考克斯-2的表达被发现在平滑肌细胞的部分肌血管和大静脉的门。支气管上皮细胞显示有限强度的考克斯-2免疫反应性。肺泡巨噬细胞和肺泡间隔细胞仅偶尔用抗Cox-2抗体染色。考克斯-1和考克斯-2在正常大鼠肺的几种细胞类型中组成型表达，但显示出明显不同的细胞定位模式。考克斯-2可能不仅与肺部炎症有关，而且还被认为与生理条件下的调节过程有关。
Prostanoid generation may proceed via both isoforms of cyclooxygenase, Cox-1 and Cox-2. Cox-1 is thought to be ubiquitously expressed, whereas Cox-2 is mostly assumed to be dynamically regulated, responding to inflammatory stimuli. The cellular localization of Cox-1 and Cox-2 in the lung, an organ with high cyclooxygenase activity, is not known. In normal rat lungs the expression and localization of Cox-1 and Cox-2 were examined with immunogold-silver staining and the RT-PCR technique. Quantitative image analysis of the staining intensity was performed by measuring mean gray values of digitized epipolarization images. Expression of both Cox-1 and Cox-2 was readily detectable in rat lungs. Cox-1 immunoreactivity localized predominantly to bronchial epithelial cells, smooth muscle cells of large hilum veins, and (with lower expression) to alveolar macrophages and pulmonary artery endothelial cells. The most intense Cox-2 staining was noted in macrophage-and mast cell-like cells, detected in close vicinity to the bronchial epithelium and in the connective tissue surrounding the vessels. In addition, strong Cox-2 expression was found in smooth muscle cells of partially muscular vessels and large veins of the hilum. Bronchial epithelial cells displayed Cox-2 immunoreactivity with limited intensity. Alveolar macrophages and alveolar septal cells were only occasionally stained with anti-Cox-2 antibodies. Both Cox-1 and Cox-2 are constitutively expressed in several cell types of normal rat lung, but display clearly different patterns of cellular localization. Cox-2 may not be related only to lung inflammation, but is suggested to be implicated in regulatory processes under physiological conditions as well.