Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in regulating stem cell proliferation and migration
Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in regulating stem cell proliferation and migration
复制标题
DOI：
10.1242/jcs.134650
复制
发表时间：
2013-11-15
影响因子：
4
通讯作者：
Chiquet-Ehrismann, Ruth
中科院分区：
文献类型：
--
作者：
Tucker, Richard P.;Ferralli, Jacqueline;Chiquet-Ehrismann, Ruth
胡须毛囊具有CD 34阳性干细胞，这些干细胞从靠近凸起处的小生境沿着玻璃膜迁移到胡须球，在那里它们参与胡须轴的形成。使用免疫组织化学，我们发现糖蛋白tenascin-C在小鼠须毛囊的纤维囊，沿着玻璃膜和周围的角蛋白-15-阴性，CD 34-阳性干细胞的小梁区域。相关的糖蛋白腱生蛋白-W存在于CD 34阳性干细胞龛中，在附近的小梁和沿着玻璃膜。生腱蛋白-W也存在于附近毛囊的神经干细胞龛中。应力纤维和黏着斑复合物的形成在CD 34阳性的晶须衍生的干细胞上培养的纤维连接蛋白抑制生腱蛋白-C和生腱蛋白-W，这是一致的，这些糖蛋白的作用，促进这些细胞的迁移，从壁龛的晶须球。生腱蛋白-C，而不是生腱蛋白-W，在体外增加胡须毛囊干细胞的增殖。因此，CD 34阳性的须毛囊干细胞龛含有腱生蛋白-C和腱生蛋白-W，这些糖蛋白可能在指导这些干细胞的迁移和增殖中发挥作用。
The whisker follicle has CD34-positive stem cells that migrate from their niche near the bulge along the glassy membrane to the whisker bulb, where they participate in the formation of the whisker shaft. Using immunohistochemistry, we found the glycoprotein tenascin-C in the fibrous capsule of mouse whisker follicles, along the glassy membrane and in the trabecular region surrounding keratin-15-negative, CD34-positive stem cells. The related glycoprotein tenascin-W is found in the CD34-positive stem cell niche, in nearby trabeculae and along the glassy membrane. Tenascin-W is also found in the neural stem cell niche of nearby hair follicles. The formation of stress fibers and focal adhesion complexes in CD34-positive whisker-derived stem cells cultured on fibronectin was inhibited by both tenascin-C and tenascin-W, which is consistent with a role for these glycoproteins in promoting the migration of these cells from the niche to the whisker bulb. Tenascin-C, but not tenascin-W, increased the proliferation of whisker follicle stem cells in vitro. Thus, the CD34-positive whisker follicle stem cell niche contains both tenascin-C and tenascin-W, and these glycoproteins might play a role in directing the migration and proliferation of these stem cells.