The influence of cytosolic phosphoglucomutase on photosynthetic carbohydrate metabolism
The influence of cytosolic phosphoglucomutase on photosynthetic carbohydrate metabolism
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DOI：
10.1007/s00425-002-0826-1
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发表时间：
2002-10-01
期刊：
影响因子：
4.3
通讯作者：
Fernie, AR
中科院分区：
文献类型：
--
作者：
Lytovchenko, A;Sweetlove, L;Fernie, AR
我们的目的。工作是研究胞质磷酸葡萄糖变位酶（cPGM; ECwww.example.com）在光合碳分配中的作用。5.4.2.2我们先前已经描述了转基因马铃薯（Solanum tuberosum cv. Desiree）系，其在35 S启动子的控制下以反义方向表达StcPGM基因。在这里，我们扩展这些线内的叶代谢的特性，检查气体交换，碳分配的特性，以及对广泛的代谢物，包括蔗糖-淀粉转换，糖酵解，克雷布斯循环和氨基酸代谢的代谢物的遗传操作的影响。在本研究中获得的数据令人惊讶地揭示，叶片的光合蔗糖合成能力在很大程度上是不变的，但这些植物显示出降低的光合作用速率，核苷酸水平的急剧降低，以及生物合成的普遍下降。我们的结论是，这些线表现出只有适度的变化，蔗糖合成，但更复杂的变化范围内的不同代谢途径。
The aim of this. work was to examine the role of cytosolic phosphoglucomutase (cPGM; EC 5.4.2.2) in photosynthetic carbon partitioning. We have previously described the generation and characterisation of the tuber metabolism of transgenic potato (Solanum tuberosum cv. Desiree) lines expressing the StcPGM gene in the antisense orientation under the control of the 35S promoter. Here we extend the characterisation of leaf metabolism within these lines, examining properties of gas exchange, carbon partitioning, and the effect of the genetic manipulation on a wide range of metabolites including metabolites of the sucrose-starch transition, glycolysis, the Krebs cycle and amino acid metabolism. The data acquired in the present study surprisingly reveal that the photosynthetic sucrose synthetic capacity of the leaves is largely unaltered but that these plants display a reduced rate of photosynthesis, a dramatic reduction in nucleotide levels, and a general decline of biosynthesis. We conclude that these lines exhibit only moderate changes in sucrose synthesis but more complex changes on a range of diverse metabolic pathways.