DIEL CHANGES IN PHASED‐DIVIDING CULTURES OF CERATIUM FURCA (DINOPHYCEAE): NUCLEOTIDE TRIPHOSPHATES, ADENYLATE ENERGY CHARGE, CELL CARBON, AND PATTERNS OF VERTICAL MIGRATION 1
DIEL CHANGES IN PHASED‐DIVIDING CULTURES OF CERATIUM FURCA (DINOPHYCEAE): NUCLEOTIDE TRIPHOSPHATES, ADENYLATE ENERGY CHARGE, CELL CARBON, AND PATTERNS OF VERTICAL MIGRATION 1
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Ceratium Furca（甲藻科）分相培养物中的昼夜变化：三磷酸核苷酸、腺苷酸电荷、细胞碳和垂直迁移模式 1
DOI：
10.1111/j.1529-8817.1979.tb00709.x
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发表时间：
1979
影响因子：
2.9
通讯作者：
D. Karl
中科院分区：
文献类型：
--
作者：
C. S. Weiler;D. Karl
测定了实验室培养的光合海洋甲藻叉角藻（Ceratium furca（Ehr.）鼓掌& Lachm.，在20 ℃下以交替的12：12 LD时间表夹带。细胞分裂开始于黑暗期的后期，约为2000年。30%的人口正在经历分裂细胞C在光照期间增加，在黑暗期间表现出线性下降，损失33%。ATP · cell−1在光照期增加，在光照期减少。40-50%在黑暗时期。细胞C和ATP的昼夜模式倾向于“缓冲”C：ATP比率的变化幅度，总体平均值为89。[GTP + UTP] · cell−1浓度在细胞周期中无明显变化趋势。细胞腺苷酸能荷在整个24小时LD周期中维持在0.8至0.9之间的值，尽管约为0.5。在12：12 LD的黑暗期间，总腺苷酸（AT= ATP + ADP + AMP）减少40%，在42 h的连续黑暗期间，ATP减少超过68%。这些数据为腺嘌呤核苷酸控制细胞代谢的理论提供了实验支持。在12：12 LD循环的侧向照射下，细胞在灯打开前不久开始集中在实验管的表面，并且在灯熄灭前不久集中在管的底部。这种模式持续了6天，在连续黑暗中，这表明垂直迁移模式是独立的趋光反应，可能是一个内源性的节奏控制下。
The diel pattern of cell division, cell carbon, adenine nucleotides and vertical migration was determined for laboratory cultures of the photosynthetic marine dinoflagellate, Ceratium furca (Ehr.) Clap. & Lachm., entrained on an alternating 12:12 LD schedule at 20 C. Cell division was initiated during the latter portion of the dark period with ca. 30% of the population undergoing division. Cell C increased during the light period and exhibited a linear decrease with a loss of 33% during the dark period. ATP · cell−1 increased during the light period and decreased by ca. 40–50% during the dark period. The diel patterns of cell C and ATP tended to “buffer” the magnitude of the change in C:ATP ratios around an overall mean value of 89. There was no obvious trend in the concentration of [GTP + UTP] · cell−1 over the cell cycle. The cellular adenylate energy charge was maintained at values between 0.8 to 0.9 throughout the 24 h LD cycle, despite a ca. 40% decrease in total adenylates (AT= ATP + ADP + AMP) during the dark period on 12:12 LD, and over a 68% decrease in ATP during 42 h of continuous darkness. These data lend experimental support to the theory of cellular metabolic control by the adenine nucleotides. With lateral illumination on 12:12 LD cycles, the cells began to concentrate at the surface of the experimental tubes shortly before the lights were turned on, and at the bottom of the tubes shortly before the lights were extinguished. This pattern continued for 6 days in continuous darkness, suggesting that the vertical migration pattern is independent of a phototactic response and may be under the control of an endogenous rhythm.