Progeny of somatic cell nuclear transfer (SCNT) pig clones are phenotypically similar to non-cloned pigs
Progeny of somatic cell nuclear transfer (SCNT) pig clones are phenotypically similar to non-cloned pigs
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DOI：
10.1089/clo.2005.7.119
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发表时间：
2005-06-01
期刊：
影响因子：
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通讯作者：
Piedrahita, JA
中科院分区：
文献类型：
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作者：
Mir, B;Zaunbrecher, G;Piedrahita, JA
对成年体细胞核产生的克隆动物进行系统研究，对于评估体细胞克隆在各种应用中的效用，包括克隆动物及其后代食品的安全性至关重要。以前，我们比较了体细胞来源的克隆猪和自然繁殖的对照猪的一系列生理和遗传参数。我们已经将我们的研究扩展到这些克隆的171个后代，看看这些表型差异是否会遗传给下一代。克隆后备母猪和自然繁殖对照组窝仔的平均窝仔数（分别为7.78 +/- 2.6和7.40 +/- 3.0;平均值+/- SD）或窝仔数变异程度（克隆和对照组窝仔的变异系数分别为33.4%和40.5%）均无差异。同样，克隆窝（51- 49%，M：F）和对照窝（59- 41%，M：F）的性别比之间也无统计学差异。在两个时间点（即，15和27周），以量化克隆对各种血液参数及其传递给下一代的影响。尽管所有性状的值的范围在不同类别之间重叠，但在15周和27周时，克隆猪和对照猪的F1后代中所有性状之间的变异性相似，只有一个例外。结合起来，我们的数据和以前在小鼠中的结果强烈支持这样的假设，即克隆动物的后代与自然繁殖动物的后代相似，因此不应该有任何与食用这些动物产品相关的风险增加。
Systematic studies of cloned animals generated from adult somatic cell nuclei are critical in assessing the utility of somatic cell cloning in various applications, including the safety of food products from cloned animals and their offspring. Previously, we compared somatic cell derived cloned pigs with naturally bred control pigs on a series of physiological and genetic, parameters. We have extended our studies to the 171 progeny of these clones to see whether these phenotypic differences are transmitted to the next generation. There were no differences in the average litter size between litters from cloned gilts and naturally bred controls (7.78 +/- 2.6 and 7.40 +/- 3.0, respectively; mean +/- SD) or in the degree of litter size variation (coefficients of variation of 33.4% and 40.5% for litters of clones and controls, respectively). Similarly there were no statistical differences between sex ratios of cloned litters (51-49%, M:F) and control litters (59-41%, M:F). Blood profiles between cloned pigs, control pigs, and their progeny were compared at two time points (i.e., 15 and 27 weeks) to quantify the effect of cloning on various blood parameters and their transmission to the next generation. Although the range of values for all traits overlapped between different classes, the variability between all the traits in F1 progeny of clones and the control pigs was similar at 15 and 27 weeks, with one exception. Combined, our data and previous results in mice strongly support the hypothesis that offspring of clones are similar to offspring of naturally bred animals, and as such there should not be any increased risks associated with consumption of products from these animals.