Gut microbiota drives the attenuation of dextran sulphate sodium-induced colitis by Huangqin decoction.
Gut microbiota drives the attenuation of dextran sulphate sodium-induced colitis by Huangqin decoction.
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肠道菌群驱动黄芩汤减轻右旋糖酐硫酸钠诱导的结肠炎
DOI：
10.18632/oncotarget.16458
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发表时间：
2017-07-25
期刊：
影响因子：
--
通讯作者：
Cao P
中科院分区：
文献类型：
--
作者：
Yang Y;Chen G;Yang Q;Ye J;Cai X;Tsering P;Cheng X;Hu C;Zhang S;Cao P
肠道微生物群包括益生菌和病原微生物，通过调节病原微生物和肠粘膜抗体的产生而参与溃疡性结肠炎（UC）。黄芩汤是《伤寒论》中记载的传统中药方剂，具有抗炎、抗氧化作用，是治疗UC的有效药物。在本研究中，我们研究了HQD是否通过改变肠道微生物群来改善葡聚糖硫酸钠（DSS）诱导的结肠炎。我们发现，HQD显着抑制结肠炎，减轻体重减轻，疾病活动指数，结肠缩短，组织损伤，和炎症细胞因子的变化诱导DSS治疗。主成分分析和主坐标分析表明，各组间差异明显，DSS和DSS+HQD组的多样性增加。使用线性判别分析效应量来确定组间差异。DSS+HQD组中乳球菌的相对丰度高于DSS组，而脱硫弧菌和螺杆菌的相对丰度降低。此外，HQD的保护作用仅在抗生素治疗的小鼠中减弱。总之，我们的研究结果表明，HQD可以通过改变肠道微生物群来改善DSS诱导的炎症。
The gut microbiota, including probiotics and pathogenic microorganisms, is involved in ulcerative colitis (UC) by regulating pathogenic microorganisms and the production of intestinal mucosal antibodies. Huangqin decoction (HQD), a traditional Chinese formula chronicled in the Shanghan lun, has been recognized as an effective drug for UC, owing to its anti-inflammatory and anti-oxidative properties. In the present study, we investigated whether HQD ameliorates dextran sulphate sodium (DSS)-induced colitis through alteration of the gut microbiota. We found that HQD significantly inhibited colitis, alleviating the loss of body weight, disease activity index, colon shortening, tissue injury, and inflammatory cytokine changes induced by DSS treatment. Principal component analysis and principal co-ordinate analysis showed an obvious difference among the groups, with increased diversity in the DSS and DSS+HQD groups. Linear discriminant analysis effect size was used to determine differences between the groups. The relative abundance of Lactococcus was higher in the DSS+HQD group than in the DSS group, whereas Desulfovibrio and Helicobacter were decreased. Furthermore, the protective effect of HQD was attenuated only in antibiotic-treated mice. In conclusion, our results suggest that HQD could ameliorate DSS-induced inflammation through alteration of the gut microbiota.