Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
Seroepidemiological survey on pigs and cattle for novel K88 (F4)-like colonisation factor detected in human enterotoxigenic Escherichia coli
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DOI：
10.1017/s0950268821002697
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发表时间：
2021-12-13
影响因子：
4.2
通讯作者：
Nishikawa Y
中科院分区：
文献类型：
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作者：
Tanimoto Y;Inoue M;Komatsu K;Odani A;Wada T;Kage-Nakadai E;Nishikawa Y
产肠毒素大肠埃希菌(ETEC)能表达多种菌毛或非菌毛定植因子(CFs)和肠毒素，是引起腹泻的重要原因。人类ETEC血清型O169：H41(O169)是世界范围内流行的ETEC的代表；该生物与Hep-2细胞表现出类似于肠聚集性大肠杆菌的大量黏附。此前，我们测定了不稳定毒力质粒pEntYN10的完整序列。除了人ETEC的代表基因CS6和人ETEC的另一个新的CF8(CFA/III)外，该质粒还包括一组编码猪ETEC新的类似于K88(F4)的Cf的基因。在本研究中，我们重点研究了类似K88的卡介苗(以下简称K88O169)，它可能会让这种微生物像原始的K88菌株一样感染家畜，导致仔猪腹泻。对来自K88O169的可能近缘粘附素的重组蛋白FaeG1和FaeG2以及典型的K88(F4)的FaeG进行了抗体检测。用重组抗原(K88O169的两个对偶FaeG1和FaeG2)进行的血清流行病学研究表明，猪(18.0%)和牛(17.1%)血清对K88O169 FaeG1和(或)FaeG2抗原具有间接ELISA反应。这些结果表明，含有K88O169粘附素的大肠杆菌可以感染多种宿主，包括猪和牛。这是国内首例家畜出现抗人ETEC K88O169抗体的报告。虽然根据CFS，人ETEC被认为与家畜的ETEC有所区别，但人畜共患病株可能隐藏在人类ETEC生物体中。应采用同一健康的概念来干预动物和人之间的ETEC感染。
Enterotoxigenic Escherichia coli (ETEC) strains that express various fimbrial or nonfimbrial colonisation factors (CFs) and enterotoxins are critical causes of diarrhoeal diseases. Human ETEC serotype O169:H41 (O169) has been a representative of epidemic ETEC worldwide; the organism shows massive adherence to HEp-2 cells similar to enteroaggregative E. coli. Previously, we determined the complete sequence of the unstable virulence plasmid, pEntYN10. The plasmid included a unique set of genes encoding a novel CF resembling K88 (F4) of porcine ETEC, in addition to CS6, a well-known representative CF of human ETEC, and another novel CF similar to CS8 (CFA/III) of human ETEC. In the present study, we focused on K88-like CF (hereafter, K88O169) that may allow the organisms to infect domestic livestock like original K88-harbouring strains that can cause diarrhoea in piglets. Samples were tested for antibodies against recombinant proteins of possible paralogous adhesins, FaeG1 and FaeG2, from K88O169 and the FaeG of typical K88 (F4). The seroepidemiological study using recombinant antigens (two paralogs FaeG1 and FaeG2 from K88O169) showed reactivity of porcine (18.0%) and bovine (17.1%) sera to K88O169 FaeG1 and/or FaeG2 antigens on indirect ELISA tests. These results suggest that E. coli with K88O169 adhesin can infect various hosts, including pigs and cattle. This is the first report of domestic livestock having antibodies to K88O169 of human ETEC. Although human ETEC had been thought to be distinguished from those of domestic animals based on CFs, zoonotic strains may conceal themselves among human ETEC organisms. The concept of One Health should be adopted to intervene in ETEC infections among animals and humans.