Chemiluminescence of a cyclometallated iridium(III) complex and its application in the detection of cysteine
Chemiluminescence of a cyclometallated iridium(III) complex and its application in the detection of cysteine
复制标题
环金属铱(III)配合物的化学发光及其在半胱氨酸检测中的应用
DOI：
10.1002/bio.1365
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发表时间：
2012-09-01
期刊：
影响因子：
2.9
通讯作者：
Zhang, Qian Feng
中科院分区：
文献类型：
--
作者：
Dong, Yong Ping;Shi, Ming Juan;Zhang, Qian Feng
本文首次报道了在高锰酸钾和草酸存在下，环化铱（Ⅲ）配合物{三[1-（2，6-二甲基苯氧基）-4-（4-氯苯基）酞嗪]铱（Ⅲ）}的化学发光。半胱氨酸对环化铱（III）配合物产生的化学发光有明显的增敏作用，使流动注射化学发光（FICL）法灵敏地检测半胱氨酸成为可能。研究了化学发光的最佳条件。在最佳条件下，测定半胱氨酸的线性范围为1.0？x？1095.0？x？一百零六？方法的检出限为6.9 μ mol/L。x？1010？mol/L时8次重复测定的相对标准偏差为1.6%。提出了化学发光的机理，并确定了发光物种为金属-配体电荷转移（MLCT）激发态的铱配合物。版权所有（c）2011约翰威利父子有限公司
Chemiluminescence (CL) of a cyclometallated iridium (III) complex {tris[1-(2,6-dimethylphenoxy)-4-(4-chlorophenyl)phthalazine]iridium(III)} in the presence of potassium permanganate and oxalic acid is reported for the first time. Cysteine exhibits sufficient enhancing effect on the CL generated from the cyclometallated iridium(III) complex, which make it possible for the sensitive detection of cysteine using a flow-injectionchemiluminescence (FICL) method. The optimum conditions for the chemiluminescence emission were investigated. Under the optimal condition, the linear range for the determination of cysteine was 1.0?x?1095.0?x?106?mol/L with a detection limit of 6.9?x?1010?mol/L. A relative standard deviation of 1.6% was obtained for eight replicate determinations. The mechanisms of CL are proposed and the emitting species was identified as the metal-to-ligand charge-transfer (MLCT) excited states of the iridium complex. Copyright (c) 2011 John Wiley & Sons, Ltd.