Metabolic regulation and optimization of oxygen supply enhance the 2,3-butanediol yield of the novel Klebsiella sp. isolate FSoil 024
Metabolic regulation and optimization of oxygen supply enhance the 2,3-butanediol yield of the novel Klebsiella sp. isolate FSoil 024
复制标题
DOI：
10.1002/biot.202100279
复制
发表时间：
2021-08-25
影响因子：
4.7
通讯作者：
Zhao, Hongxin
中科院分区：
文献类型：
--
作者：
Chu, Wanying;Jiang, Ke;Zhao, Hongxin
背景生物2，3-丁二醇(2，3-BDO)是一种高附加值的化合物，既可用作液体燃料，又可用作平台化学品。生物生产2，3-BDO是一种环境友好的选择。方法和结果新型克雷伯氏菌的3种重组衍生物。FSoil 024(WT)菌株的构建采用不同的策略，包括利用lambda-Red同源重组技术删除乳酸脱氢酶、高表达小非编码RNA RyhB以及两者的结合。摇瓶发酵24 h后，2，3-BDO产量提高了61.3%-79%，其中WT-Delta LDH/ryhB的2，3-BDO产量最高，达42.36 mM。葡萄糖是WT-Delta LDH/ryhB发酵生产2，3-BDO的最佳碳源。此外，较高的含氧量有利于理想产物的合成。在氧气浓度为70%(V：V‘=O-2：(O-2+N-2))的条件下，WT和WT-Delta LDH/ryhB的最大2，3-BDO产率分别比对照组提高23.3%和52.5%。结论和意义根据本研究，乳酸脱氢酶的缺失、RyhB的过表达和供氧的控制对新分离的克雷伯氏菌的细胞生长、2，3-BDO产量和细胞代谢有很大的影响。FSoil 024.这项工作也将为促进2，3-BDO的生物合成在工业上的应用提供借鉴。
Background Biogenic 2,3-butanediol (2,3-BDO) is a high-value-added compound that can be used as a liquid fuel and a platform chemical. Bioproduction of 2,3-BDO is an environmentally friendly choice. Method and Results Three recombinant derivatives of the novel Klebsiella sp. isolate FSoil 024 (WT) were constructed via different strategies including deletion of lactate dehydrogenase by lambda-Red homologous recombination technology, overexpression of the small-noncoding RNA RyhB and a combination of both. The 2,3-BDO productivity of the mutants increased by 61.3%-79%, and WT-Delta ldh/ryhB displayed the highest 2,3-BDO yield of 42.36 mM after 24 h of shake-flask fermentation. Glucose was shown as the best carbon source for 2,3-BDO production by WT-Delta ldh/ryhB. In addition, higher oxygenation was favorable for ideal product synthesis. The maximal 2,3-BDO yield of WT and WT-Delta ldh/ryhB were increased by 23.3% and 52.5% respectively compared to the control group in the presence of 70% oxygen (V:V' = O-2:(O-2 + N-2)). Conclusion and Implications According to the present study, deletion of lactate dehydrogenase, RyhB overexpression, and manipulation of oxygen supply showed great impacts on cell growth, 2,3-BDO productivity and cellular metabolism of the novel isolated strain Klebsiella sp. FSoil 024. This work would also provide insights for promoting 2,3-BDO biosynthesis for industrial applications.