Lysozyme mediated calcium carbonate mineralization.
Lysozyme mediated calcium carbonate mineralization.
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DOI：
10.1016/j.jcis.2008.12.055
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发表时间：
2009-04
影响因子：
9.9
通讯作者：
Xiaoqiang Wang;Hai Sun;Yongqing Xia;Cuixia Chen;Hai Xu;H. Shan;J. Lu
中科院分区：
文献类型：
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作者：
Xiaoqiang Wang;Hai Sun;Yongqing Xia;Cuixia Chen;Hai Xu;H. Shan;J. Lu
溶菌酶是蛋清蛋白的主要成分，据推测它参与了鸟类蛋壳的钙化。然而，它在蛋壳形成过程中的详细作用还不清楚。本文采用FTIR、XRD、SEM等方法研究了溶菌酶对caco3沉淀的影响。在特制的气室中，由（NH4） 2co3蒸气扩散到cac2水溶液中产生沉淀。在没有溶菌酶的情况下，在研究的前3 ~ 12 h结晶过程中，沉淀主要由六方晶片及其球形聚集体组成，（001）晶面在六方晶方向上表现良好。相反，方解石在溶菌酶存在的情况下更容易沉淀，且效果与溶菌酶浓度成正比。此外，方解石的（110）面除了表达常见的（104）面外，形态修饰也依赖于溶菌酶的浓度。我们将这些现象归因于铵离子和溶菌酶在不同晶面上的选择性吸附。我们的研究结果清楚地揭示了溶菌酶在caco3沉淀中的浓度和面依赖性作用。这与子宫液中溶菌酶的丰度一起，意味着它对蛋壳形成初期方解石的分层结构的直接贡献。
Lysozyme, a major component of egg white proteins, has been speculated to participate in the calcification of avian eggshells. However, its detailed role during the eggshell formation is not well understood. In this work, the influence of lysozyme on the precipitation of CaCO3has been investigated using a combined study of FTIR, XRD, and SEM. The precipitation was produced from (NH4)2CO3vapor diffusion into CaCl2aqueous solution using a specially built chamber. In the absence of lysozyme, hexagonal platelets of vaterite and their spherical aggregates dominated the precipitates during the first 3–12 h crystallization period studied, with the (001) crystal face well expressed in the hexagonal direction. In contrast, calcite was favored to precipitate in the presence of lysozyme during the same period and the effect was found to be proportional to lysozyme concentration. Furthermore, the (110) face of calcite was expressed in addition to the common (104) face, and the morphological modification was also lysozyme concentration dependent. We attributed these phenomena to the selective adsorption of ammonium ions and lysozyme onto different crystal faces. Our findings have clearly revealed the concentration and face dependent role of lysozyme in CaCO3precipitation. This, together with the abundance of lysozyme in the uterine fluid, implies its direct contribution to the hierarchical structures of calcite during the initial stage of eggshell formation.