Antibacterial Synergisms of Ji Xue Teng, Spatholobus suberectus, Extract and Selected Antibiotics against Streptococcus agalactiae from Nile Tilapia, Oreochromis niloticus (L.), In Vitro and In Vivo
Antibacterial Synergisms of Ji Xue Teng, Spatholobus suberectus, Extract and Selected Antibiotics against Streptococcus agalactiae from Nile Tilapia, Oreochromis niloticus (L.), In Vitro and In Vivo
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DOI：
10.1111/jwas.12516
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发表时间：
2018-12-01
影响因子：
2.8
通讯作者：
Zhou, Yong-Can
中科院分区：
文献类型：
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作者：
Guo, Wei-Liang;Wang, Wen-Hui;Zhou, Yong-Can
无乳链球菌感染给罗非鱼生产造成了严重的经济损失。为寻找治疗无乳链球菌感染的有效策略，本研究采用改良琼脂扩散法筛选鸡血藤、鸡血藤、鸡血藤提取物与部分抗生素的抗菌协同作用。优化了猪链球菌提取物与所选抗生素的组合比例，观察了它们对无乳链霉菌感染尼罗罗非鱼的影响，并测定了它们的抗菌谱。结果表明，猪链霉菌提取物与硫酸链霉素对无乳链球菌有协同抗菌作用，最佳配比为42.5：1(m：m)。猪链霉菌提取物的最低抑菌浓度由27.84 mg/m L降至5.95 mg/m L，硫酸链霉素由0.66 m g/m L降至0.13 m g/m L。给感染尼罗罗非鱼投喂含0.520%猪链霉菌提取物、0.013%硫酸链霉素或两者合用的饲料后，其相对存活率分别为52.6%、13.9%和80.5%。这些结果表明猪链霉菌提取物与硫酸链霉素在体内外具有协同抗菌作用。
Streptococcus agalactiae infection causes severe economic losses in tilapia production. To find out efficacious strategies for curing S. agalactiae infections, this study screened the antibacterial synergisms between Ji Xue Teng, Spatholobus suberectus, extract and selected antibiotics via a modified agar well diffusion method. We optimized a combined ratio of S. suberectus extract and the selected antibiotic, observed their effects on the infections with S. agalactiae on Nile tilapia, Oreochromis niloticus (L.), and determined their antibacterial spectra. The results showed that S. suberectus extract and streptomycin sulfate exhibited antibacterial synergism against S. agalactiae, with an optimal ratio of 42.5:1 (m : m). The minimal inhibitory concentration of S. suberectus extract was reduced from 27.84 to 5.95 mg/mL, and a reduction of streptomycin sulfate from 0.66 to 0.13 mg/mL was noted. After providing infected tilapia, O. niloticus (L.), with feedstuffs containing 0.520% S. suberectus extract only, 0.013% streptomycin sulfate only, or in combination, the relative percent of survival was 52.6, 13.9, and 80.5%, respectively. These results indicate an antibacterial synergism between S. suberectus extract and streptomycin sulfate in vitro and in vivo.