The MTE, a new core promoter element for transcription by RNA polymerase II
The MTE, a new core promoter element for transcription by RNA polymerase II
复制标题
DOI：
10.1101/gad.1193404
复制
发表时间：
2004-07-01
影响因子：
10.5
通讯作者：
Kadonaga, JT
中科院分区：
文献类型：
--
作者：
Lim, CY;Santoso, B;Kadonaga, JT
核心启动子是大量调节因子网络的最终靶标，这些调节因子有助于RNA聚合酶II启动转录。在这里，我们描述了MTE（基序10元件），一个新的核心启动子元件，似乎是从果蝇到人类的保守。当MTE精确地位于起始（Inr）元件中相对于A（+1）的+18至+27位时，它通过RNA聚合酶II促进转录。相对于A（+1）+18至+22的MTE序列对于基础转录很重要，+18至+27的区域足以赋予异源核心启动子MTE活性。MTE需要Inr，但其功能独立于TATA盒和DPE。值得注意的是，TATA盒或DPE突变后转录活性的损失可以通过添加MTE来补偿。此外，MTE与TATA盒以及DPE表现出强的协同作用。这些研究结果表明，MTE是一种新的下游核心启动子元件，是重要的RNA聚合酶II的转录。
The core promoter is the ultimate target of the vast network of regulatory factors that contribute to the initiation of transcription by RNA polymerase II. Here we describe the MTE (motif ten element), a new core promoter element that appears to be conserved from Drosophila to humans. The MTE promotes transcription by RNA polymerase II when it is located precisely at positions +18 to +27 relative to A(+1) in the initiator (Inr) element. MTE sequences from +18 to +22 relative to A(+1) are important for basal transcription, and a region from +18 to +27 is sufficient to confer MTE activity to heterologous core promoters. The MTE requires the Inr, but functions independently of the TATA-box and DPE. Notably, the loss of transcriptional activity upon mutation of a TATA-box or DPE can be compensated by the addition of an MTE. In addition, the MTE exhibits strong synergism with the TATA-box as well as the DPE. These findings indicate that the MTE is a novel downstream core promoter element that is important for transcription by RNA polymerase II.