The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana
The Rep proteins encoded by alphasatellites restore expression of a transcriptionally silenced green fluorescent protein transgene in Nicotiana benthamiana
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DOI：
10.1007/s13337-017-0413-5
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发表时间：
2019-03-01
期刊：
影响因子：
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通讯作者：
Briddon, Rob W.
中科院分区：
文献类型：
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作者：
Abbas, Qamar;Amin, Imran;Briddon, Rob W.
阿尔法卫星是与双子星病毒相关的非必要的类似卫星的部件。双病毒感染一颗α卫星的确切选择优势尚不清楚。利用携带转录沉默绿色荧光蛋白（GFP）转基因的benthamiana Nicotiana 16-TGS (16-TGS)，研究了棉花叶片卷曲Multan alphasatellite （CLCuMuA）编码复制相关蛋白（Rep）抑制TGS的能力。用携带CLCuMuA Rep的重组马铃薯病毒X （PVX）载体接种16-TGS植株，可恢复GFP的表达。Northern blot分析证实，观察到的GFP荧光与GFP mRNA积累有关。用PVX载体接种含有另外六种Rep蛋白（由遗传上不同的α卫星编码）的PVX载体，同样可以使16-TGS植株恢复GFP表达。这些结果表明，alphasatellites编码的Rep可以恢复benthamiana中转录沉默的GFP转基因的表达，表明alphasatellites参与克服宿主防御。
Alphasatellites are non-essential satellite-like components associated with geminiviruses. The precise selective advantage to a geminivirus infection of an alphasatellite remains unclear. The ability of the cotton leaf curl Multan alphasatellite (CLCuMuA)-encoded replication-associated protein (Rep) to suppress TGS was investigated by using Nicotiana benthamiana line 16-TGS (16-TGS) harbouring a transcriptionally silenced green fluorescent protein (GFP) transgene. Inoculation of 16-TGS plants with a recombinant Potato virus X (PVX) vector carrying CLCuMuA Rep resulted in restoration of GFP expression. Northern blot analysis confirmed that the observed GFP fluorescence was associated with GFP mRNA accumulation. Inoculation with PVX vectors harbouring a further six Rep proteins, encoded by genetically distinct alphasatellites, were similarly shown to result in 16-TGS plants with restored GFP expression. These results indicate that the alphasatellite-encoded Rep can restore the expression of a transcriptionally silenced GFP transgene in N. benthamiana, indicating that alphasatellites are involved in overcoming host defence.