Simply and Cheaply Prepared Liposomal Membrane for Nanocarriers: High Encapsulation Efficiency Based on Broad Regulation of Surface Charges and pH-Switchable Performance
Simply and Cheaply Prepared Liposomal Membrane for Nanocarriers: High Encapsulation Efficiency Based on Broad Regulation of Surface Charges and pH-Switchable Performance
复制标题
DOI：
10.1021/acs.biomac.3c00679
复制
发表时间：
2023-11-16
影响因子：
6.2
通讯作者：
Wang，Xu
中科院分区：
文献类型：
--
作者：
Wu，Jiangjie;Zhang，Xin;Wang，Xu
纳米粒子的zeta电位影响它们在体内的分布和代谢，以及它们与不同电荷的药物的相互作用，从而改变治疗效果和安全性。本文利用阳离子壳聚糖（CS）和阴离子透明质酸（HA）的质子竞争调控脂质体的外部电荷。脂质体膜的电荷调节一般是通过调节脂质体内带电荷的脂质比例来完成的（如阳离子DOTAP或阴离子DOPS），其稳定性由阳离子壳聚糖（CS）或阴离子透明质酸（HA）的涂层材料来维持。一系列纳米颗粒可以通过调节表面电荷来响应ph刺激。此外，CS和HA包被的脂质体的大小保持在一个狭窄的范围内。体外细胞毒性试验表明，纳米载体是安全的，含有抗肿瘤药物的纳米颗粒在肿瘤治疗中是有效的。考虑到脂质体具有不同的外表面电荷，可以针对不同的治疗目的。本文报道的具有高包封率和特定释放周期的调节脂质体表面电荷的策略可以为药物和其他大分子进入人体提供一个多功能的载体平台。
The zeta potential of nanoparticles impacts their distribution and metabolism in the body as well as their interaction with medications of varying charges, hence altering therapeutic efficacy and safety. In this paper, the external charges of liposomes were regulated by utilizing a simple and economical method based on competition for protons of cationic chitosan (CS) and anion hyaluronic acid (HA). The charge regulation of a liposomal membrane is generally accomplished by adjusting the ratio of charged lipids within a liposome (e.g., cationic DOTAP or anionic DOPS), the stability of which was maintained by the coating materials of cationic chitosan (CS) or anion hyaluronic acid (HA). A series of nanoparticles could respond to pH-stimulation with adjustable surface charge. Moreover, the sizes of liposomes coated with CS and HA remain within a narrow range. In vitro cytotoxicity tests revealed that the nanocarriers were safe, and the nanoparticles containing antitumor medicines were efficient in tumor therapy. Considering liposomes with different external surface charges could be aimed at diverse therapy purposes. The strategies for regulating liposomal surface charges with high encapsulation rates and certain release cycles reported here could provide a versatile platform as carriers for the delivery of drugs and other macromolecules into human bodies.