One, two, infinity: Genomes filled with aptamers
One, two, infinity: Genomes filled with aptamers
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DOI：
10.1016/s1074-5521(02)00286-7
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发表时间：
2002-12-01
影响因子：
--
通讯作者：
Singer, B
中科院分区：
文献类型：
--
作者：
Gold, L;Brody, E;Singer, B
第一，第二，催化的现代基础，功能的同时划分可能使辅因子不含寡核苷酸。单个细胞通过监测其直接的环境核苷酸（作为催化所需蛋白质的辅助物）来限制不必要代谢能力的产生，并使寡核苷酸游离并适当地调节基因表达。这可能导致更高的效率和能量利用率来编码蛋白质。白色预测了一个早期的世界，在那里“形状和磁带”被发现在同一anzation，同时允许快速反应的环境变化。细胞会对单个代谢分子的变化做出反应，劳动分工导致了更简单的密码（只有四个简单的核苷酸）和更好的催化剂，如碳源和氮源或维生素，通过调节合成酶（具有辅因子的蛋白质，可以自由进化或不进化，但不再受寡核苷酸或特定代谢物利用规则的约束）所必需的基因表达。奇怪的是，虽然最终的化学信号，其中细胞recleotides）.白色对我们现在所称的“RNA世界”有着深刻的见解，尽管他并没有声称海绵就是代谢物本身，直到现在，调节因子通常是蛋白质。这一观点和吉尔伯特一样具有决定性。因为E.大肠杆菌内的适体活性的一个罗恩Breaker和他的同事[1，2]发表了两篇论文，证明翻译mRNA的调节是在表达系统的背景下，参与合成的辅因子，Breaker interthrough直接相互作用的小分子代谢物与mRNA编码的蛋白质所必需的，为他们的up-prets他的发现作为一个世界的反映。我们爱哈罗德B。白色和罗恩布雷克的工作，采取或利用。在第一篇论文中，btu B前导序列，5到翻译起始结构域，但我们想知道，对于这一讨论来说，残留物是否不如对协调的btu B的连续选择重要，它直接与辅酶B12相互作用，以这种方式导致RNA的结构变化，在过去的几十亿年里，在哈罗德B之后，这种结构变化隔离了调节。白色的辅因子已经离开了他们以前的寡核苷酸翻译起始结构域从核糖体检查。btuB基因编码B12核苷酸归巢的受体。
One, Two the modern basis of catalysis, a contemporaneous partitioning of function could have left cofactors free of oligo-Individual cells limit production of unnecessary metabolic capacities by monitoring their immediate environ- nucleotides (to function as aids to the proteins that needed them for catalysis) and left oligonucleotides free ment and regulating gene expression appropriately. This presumably results in greater efficiency and energy utili- to encode proteins. White predicted an earlier world in which “shapes and tapes” were found in the same anzation while allowing rapid response to environmental changes. Cells respond to changes in individual metab- cient molecules, and that division of labor led to a simpler code (only four simple nucleotides) and better cataolites such as carbon and nitrogen sources or vitamins by regulating expression of genes necessary for synthe- lysts (proteins with cofactors, which were free to evolve or not, but were no longer bound by the rules for oligonusis or utilization of the specific metabolite. Curiously, although the ultimate chemical signal to which cells re- cleotides). White had a substantial vision into what we now call the “RNA world,” although he did not claim the spond is the metabolite itself, until now the regulatory factotum usually has been a protein. idea as conclusively as did Gilbert [8]. Because these two examples in E. coli of aptamer activity within an Ron Breaker and his colleagues [1, 2] have published two papers that demonstrate regulation of translation mRNA are in the context of expression systems that participate in the synthesis of cofactors, Breaker interthrough direct interaction of a small molecule metabolite with mRNAs encoding proteins necessary for their up- prets his findings as a reflection of the world that was. We love Harold B. White’s and Ron Breaker’s work, take or utilization. In the first paper, the btuB leader sequence, 5 to the translational initiation domain for yet we wonder if remnants are less important to this discussion than continuous selection for coordinated btuB, interacts directly with coenzyme B12 in such a way as to cause structural changes to the RNA that sequester regulation over the last several billion years, well after Harold B. White’s cofactors had left their previous oligothe translational initiation domain from ribosome inspection. The btuB gene encodes the receptor by which B12 nucleotide homes.